doi:10.1111/apha.12498engLatorre, E.Layunta, E.Gimeno, A.Grasa, L.Castro, M.García, S.Gros, B.Arruebo, M.P.Alcalde, A.I.Mesonero, J.E.Oxidative stress balance between pro- and anti-inflammatory factors in human intestinal epithelial cellsART-2015-99902Background: Oxidative stress plays a key role in the development of intestinal inflammatory diseases. Several pro-inflammatory mediators may generate oxidation products that exacerbate the inflammatory damage. Gastrointestinal molecules, like serotonin (5- HT), adenosine and melatonin, which are involved in intestinal physiology, have also been described as intestinal pro-inflammatory factors; whereas IL-10, a known anti-inflammatory cytokine, has also been implicated in intestinal pathophysiology. Aim: The aim of this study was to analyze the contribution of pro-inflammatory and anti-inflammatory molecules to oxidative stress balance, as well as to assess their effect on cellular antioxidant enzymes activity, in intestinal epithelial cells. Methods: Caco-2 cells were treated with the different molecules, and the oxidative stress was determined by measuring lipid peroxidation (MDA+4HDA) and protein carbonyl levels. The activity of the anti-oxidant enzymes (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) was also analyzed in treated cells. Results: Pro-inflammatory factors (5-HT, adenosine, melatonin and TNFa) increased oxidative damage in both lipids and proteins. Theses molecules, except melatonin, also inhibited the activity of antioxidant enzymes. With regard to IL-10, this cytokine was not shown to alter cellular oxidative damage, but was able to reduce the oxidative damage g by pro-inflamatory factors, and to restore their effects on anti-oxidant enxymes activities. Unexpectedly, IL-10, together with melatonin, was found to increase the antioxidant activity above the control. Conclusions: The anti-oxidant effect of IL-10 emphasizes the role of this cytokine as a potential therapy for the treatment of intestinal inflammation induced by pro-inflammatory molecules.2015http://zaguan.unizar.es/record/6183210.1111/apha.12498http://zaguan.unizar.es/record/61832oai:zaguan.unizar.es:61832info:eu-repo/grantAgreement/ES/DGA/ARAINF-012-2008info:eu-repo/grantAgreement/ES/DGA/B022-13info:eu-repo/grantAgreement/ES/DGA/B105-11info:eu-repo/grantAgreement/ES/DGA/B61info:eu-repo/grantAgreement/ES/MICINN-FEDER/BFU2009-08149info:eu-repo/grantAgreement/ES/MICINN-FEDER/BFU2010-18971Acta Physiologica 214, Suppl. 701 (2015), 30 [14]byhttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccess