61832 20240122154813.0 doi 10.1111/apha.12498 sideral 99902 ART-2015-99902 eng Latorre, E. Oxidative stress balance between pro- and anti-inflammatory factors in human intestinal epithelial cells 2015 Access copy available to the general public Unrestricted Background: Oxidative stress plays a key role in the development of intestinal inflammatory diseases. Several pro-inflammatory mediators may generate oxidation products that exacerbate the inflammatory damage. Gastrointestinal molecules, like serotonin (5- HT), adenosine and melatonin, which are involved in intestinal physiology, have also been described as intestinal pro-inflammatory factors; whereas IL-10, a known anti-inflammatory cytokine, has also been implicated in intestinal pathophysiology. Aim: The aim of this study was to analyze the contribution of pro-inflammatory and anti-inflammatory molecules to oxidative stress balance, as well as to assess their effect on cellular antioxidant enzymes activity, in intestinal epithelial cells. Methods: Caco-2 cells were treated with the different molecules, and the oxidative stress was determined by measuring lipid peroxidation (MDA+4HDA) and protein carbonyl levels. The activity of the anti-oxidant enzymes (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) was also analyzed in treated cells. Results: Pro-inflammatory factors (5-HT, adenosine, melatonin and TNFa) increased oxidative damage in both lipids and proteins. Theses molecules, except melatonin, also inhibited the activity of antioxidant enzymes. With regard to IL-10, this cytokine was not shown to alter cellular oxidative damage, but was able to reduce the oxidative damage g by pro-inflamatory factors, and to restore their effects on anti-oxidant enxymes activities. Unexpectedly, IL-10, together with melatonin, was found to increase the antioxidant activity above the control. Conclusions: The anti-oxidant effect of IL-10 emphasizes the role of this cytokine as a potential therapy for the treatment of intestinal inflammation induced by pro-inflammatory molecules. info:eu-repo/grantAgreement/ES/DGA/ARAINF-012-2008 info:eu-repo/grantAgreement/ES/DGA/B022-13 info:eu-repo/grantAgreement/ES/DGA/B105-11 info:eu-repo/grantAgreement/ES/DGA/B61 info:eu-repo/grantAgreement/ES/MICINN-FEDER/BFU2009-08149 info:eu-repo/grantAgreement/ES/MICINN-FEDER/BFU2010-18971 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.066 2015 PHYSIOLOGY 13 / 83 = 0.157 2015 Q1 T1 1.654 2015 Physiology 2015 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Layunta, E. Universidad de Zaragoza (orcid)0000-0001-5573-6144 Gimeno, A. Universidad de Zaragoza (orcid)0000-0003-2373-6056 Grasa, L. Universidad de Zaragoza (orcid)0000-0002-5306-9365 Castro, M. Universidad de Zaragoza (orcid)0000-0001-8584-3979 García, S. Universidad de Zaragoza (orcid)0000-0003-3970-5457 Gros, B. Arruebo, M.P. Universidad de Zaragoza (orcid)0000-0002-2114-0577 Alcalde, A.I. Mesonero, J.E. Universidad de Zaragoza (orcid)0000-0003-4758-3998 1005 410 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Fisiología 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 214, Suppl. 701 (2015), 30 [14] Acta Physiol. Acta Physiologica 1748-1708 36654 http://zaguan.unizar.es/record/61832/files/texto_completo.pdf Versión publicada 119323 http://zaguan.unizar.es/record/61832/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:61832 articulos driver 2024-01-22-15:31:36 ARTICLE