61835 20231215090947.0 doi 10.1038/ncomms5450 sideral 98809 ART-2014-98809 eng Ito, S. Loss of neuronal 3d chromatin organization causes transcriptional and behavioural deficits related to serotonergic dysfunction 2014 Access copy available to the general public Unrestricted The interior of the neuronal cell nucleus is a highly organized three-dimensional (3D) structure where regions of the genome that are linearly millions of bases apart establish sub-structures with specialized functions. To investigate neuronal chromatin organization and dynamics in vivo, we generated bitransgenic mice expressing GFP-tagged histone H2B in principal neurons of the forebrain. Surprisingly, the expression of this chimeric histone in mature neurons caused chromocenter declustering and disrupted the association of heterochromatin with the nuclear lamina. The loss of these structures did not affect neuronal viability but was associated with specific transcriptional and behavioural deficits related to serotonergic dysfunction. Overall, our results demonstrate that the 3D organization of chromatin within neuronal cells provides an additional level of epigenetic regulation of gene expression that critically impacts neuronal function. This in turn suggests that some loci associated with neuropsychiatric disorders may be particularly sensitive to changes in chromatin architecture. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 11.47 2014 MULTIDISCIPLINARY SCIENCES 3 / 57 = 0.053 2014 Q1 T1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Magalska, A. Alcaraz-Iborra, M. Lopez-Atalaya, J. Rovira, V. Contreras-Moreira, B. (orcid)0000-0002-5462-907X Lipinski, M. Olivares, R. Martinez-Hernandez, J. Ruszczycki, B. Lujan, R. Geijo-Barrientos, E. Wilczynski, G.M. Barco, A. 5 (2014), 4450 [14 pp] Nature communications 2041-1723 6188991 http://zaguan.unizar.es/record/61835/files/texto_completo.pdf Versión publicada 76857 http://zaguan.unizar.es/record/61835/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:61835 articulos driver 2023-12-15-08:57:02 ARTICLE