000063155 001__ 63155 000063155 005__ 20190709135511.0 000063155 0247_ $$2doi$$a10.1186/s12989-017-0222-4 000063155 0248_ $$2sideral$$a101891 000063155 037__ $$aART-2017-101891 000063155 041__ $$aeng 000063155 100__ $$aPérez-Hernández, Marta 000063155 245__ $$aMultiparametric analysis of anti-proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells, biodistribution and toxicity in vivo 000063155 260__ $$c2017 000063155 5060_ $$aAccess copy available to the general public$$fUnrestricted 000063155 5203_ $$aBackground: The special physicochemical properties of gold nanoprisms make them very useful for biomedical applications including biosensing and cancer therapy. However, it is not clear how gold nanoprisms may affect cellular physiology including viability and other critical functions. We report a multiparametric investigation on the impact of gold-nanoprisms on mice and human, transformed and primary cells as well as tissue distribution and toxicity in vivo after parental injection. Methods: Cellular uptake of the gold-nanoprisms (NPRs) and the most crucial parameters of cell fitness such as generation of reactive oxygen species (ROS), mitochondria membrane potential, cell morphology and apoptosis were systematically assayed in cells. Organ distribution and toxicity including inflammatory response were analysed in vivo in mice at 3 days or 4 months after parental administration. Results: Internalized gold-nanoprisms have a significant impact in cell morphology, mitochondrial function and ROS production, which however do not affect the potential of cells to proliferate and form colonies. In vivo NPRs were only detected in spleen and liver at 3 days and 4 months after administration, which correlated with some changes in tissue architecture. However, the main serum biochemical markers of organ damage and inflammation (TNFa and IFN¿) remained unaltered even after 4 months. In addition, animals did not show any macroscopic sign of toxicity and remained healthy during all the study period. Conclusion: Our data indicate that these gold-nanoprisms are neither cytotoxic nor cytostatic in transformed and primary cells, and suggest that extensive parameters should be analysed in different cell types to draw useful conclusions on nanomaterials safety. Moreover, although there is a tendency for the NPRs to accumulate in liver and spleen, there is no observable negative impact on animal health. 000063155 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-1-R$$9info:eu-repo/grantAgreement/ES/MINECO/MAT2011-26851-CO2-02$$9info:eu-repo/grantAgreement/ES/MINECO/MAT2011-26851-CO2-01$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/SAF2011-25390$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 754609-HOTFLOW$$9info:eu-repo/grantAgreement/EC/H2020/754609/EU/A Photothermal Lateral Flow Test for Visual Point of Care Detection/HOTFLOW$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 660228-HyHeat$$9info:eu-repo/grantAgreement/EC/H2020/660228/EU/Profiling gene expression in Hydra vulgaris following Gold Nanoparticle-mediated hyperthermia/HyHeat$$9info:eu-repo/grantAgreement/EC/FP7/239931/EU/Multifunctional Magnetic Nanoparticles: Towards Smart Drugs Design/NANOPUZZLE$$9info:eu-repo/grantAgreement/ES/CSIC/PIE-201460I019$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-2-R 000063155 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000063155 590__ $$a6.105$$b2017 000063155 591__ $$aTOXICOLOGY$$b5 / 94 = 0.053$$c2017$$dQ1$$eT1 000063155 592__ $$a2.253$$b2017 000063155 593__ $$aHealth, Toxicology and Mutagenesis$$c2017$$dQ1 000063155 593__ $$aToxicology$$c2017$$dQ1 000063155 593__ $$aMedicine (miscellaneous)$$c2017$$dQ1 000063155 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000063155 700__ $$0(orcid)0000-0002-2861-2469$$aMoros, María 000063155 700__ $$0(orcid)0000-0002-4170-7999$$aStepien, Grazyna 000063155 700__ $$0(orcid)0000-0002-1477-5259$$aDel Pino, Pablo 000063155 700__ $$aMenao, Sebastián 000063155 700__ $$0(orcid)0000-0003-3792-287X$$aHeras, Marcelo de las$$uUniversidad de Zaragoza 000063155 700__ $$0(orcid)0000-0002-9730-2210$$aArias, Maykel 000063155 700__ $$0(orcid)0000-0003-4848-414X$$aMitchell, Scott G.$$uUniversidad de Zaragoza 000063155 700__ $$aPelaz, Beatriz 000063155 700__ $$aGálvez, Eva M. 000063155 700__ $$aFuente, Jesús M. de la 000063155 700__ $$0(orcid)0000-0003-0154-0730$$aPardo, Julián$$uUniversidad de Zaragoza 000063155 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000063155 7102_ $$11008$$2566$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Inmunología 000063155 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica 000063155 773__ $$g14, 1 (2017), [20 pp]$$pPart fibre toxicol.$$tPARTICLE AND FIBRE TOXICOLOGY$$x1743-8977 000063155 8564_ $$s6429475$$uhttps://zaguan.unizar.es/record/63155/files/texto_completo.pdf$$yVersión publicada 000063155 8564_ $$s89595$$uhttps://zaguan.unizar.es/record/63155/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000063155 909CO $$ooai:zaguan.unizar.es:63155$$particulos$$pdriver 000063155 951__ $$a2019-07-09-11:51:52 000063155 980__ $$aARTICLE