63155 20190709135511.0 doi 10.1186/s12989-017-0222-4 sideral 101891 ART-2017-101891 eng Pérez-Hernández, Marta Multiparametric analysis of anti-proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells, biodistribution and toxicity in vivo 2017 Access copy available to the general public Unrestricted Background: The special physicochemical properties of gold nanoprisms make them very useful for biomedical applications including biosensing and cancer therapy. However, it is not clear how gold nanoprisms may affect cellular physiology including viability and other critical functions. We report a multiparametric investigation on the impact of gold-nanoprisms on mice and human, transformed and primary cells as well as tissue distribution and toxicity in vivo after parental injection. Methods: Cellular uptake of the gold-nanoprisms (NPRs) and the most crucial parameters of cell fitness such as generation of reactive oxygen species (ROS), mitochondria membrane potential, cell morphology and apoptosis were systematically assayed in cells. Organ distribution and toxicity including inflammatory response were analysed in vivo in mice at 3 days or 4 months after parental administration. Results: Internalized gold-nanoprisms have a significant impact in cell morphology, mitochondrial function and ROS production, which however do not affect the potential of cells to proliferate and form colonies. In vivo NPRs were only detected in spleen and liver at 3 days and 4 months after administration, which correlated with some changes in tissue architecture. However, the main serum biochemical markers of organ damage and inflammation (TNFa and IFN¿) remained unaltered even after 4 months. In addition, animals did not show any macroscopic sign of toxicity and remained healthy during all the study period. Conclusion: Our data indicate that these gold-nanoprisms are neither cytotoxic nor cytostatic in transformed and primary cells, and suggest that extensive parameters should be analysed in different cell types to draw useful conclusions on nanomaterials safety. Moreover, although there is a tendency for the NPRs to accumulate in liver and spleen, there is no observable negative impact on animal health. info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-1-R info:eu-repo/grantAgreement/ES/MINECO/MAT2011-26851-CO2-02 info:eu-repo/grantAgreement/ES/MINECO/MAT2011-26851-CO2-01 info:eu-repo/grantAgreement/ES/MINECO-FEDER/SAF2011-25390 This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 754609-HOTFLOW info:eu-repo/grantAgreement/EC/H2020/754609/EU/A Photothermal Lateral Flow Test for Visual Point of Care Detection/HOTFLOW This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 660228-HyHeat info:eu-repo/grantAgreement/EC/H2020/660228/EU/Profiling gene expression in Hydra vulgaris following Gold Nanoparticle-mediated hyperthermia/HyHeat info:eu-repo/grantAgreement/EC/FP7/239931/EU/Multifunctional Magnetic Nanoparticles: Towards Smart Drugs Design/NANOPUZZLE info:eu-repo/grantAgreement/ES/CSIC/PIE-201460I019 info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-2-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 6.105 2017 TOXICOLOGY 5 / 94 = 0.053 2017 Q1 T1 2.253 2017 Health, Toxicology and Mutagenesis 2017 Q1 Toxicology 2017 Q1 Medicine (miscellaneous) 2017 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion (orcid)0000-0002-2861-2469 Moros, María (orcid)0000-0002-4170-7999 Stepien, Grazyna (orcid)0000-0002-1477-5259 Del Pino, Pablo Menao, Sebastián (orcid)0000-0003-3792-287X Heras, Marcelo de las Universidad de Zaragoza (orcid)0000-0002-9730-2210 Arias, Maykel (orcid)0000-0003-4848-414X Mitchell, Scott G. Universidad de Zaragoza Pelaz, Beatriz Gálvez, Eva M. Fuente, Jesús M. de la (orcid)0000-0003-0154-0730 Pardo, Julián Universidad de Zaragoza 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1008 566 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Inmunología 2013 765 Universidad de Zaragoza Dpto. Química Orgánica Área Química Orgánica 14, 1 (2017), [20 pp] Part fibre toxicol. PARTICLE AND FIBRE TOXICOLOGY 1743-8977 6429475 http://zaguan.unizar.es/record/63155/files/texto_completo.pdf Versión publicada 89595 http://zaguan.unizar.es/record/63155/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:63155 articulos driver 2019-07-09-11:51:52 ARTICLE