63224 20200428120341.0 doi 10.2527/jas.2016.1286 sideral 98852 ART-2017-98852 eng Barranco, I. Profile and reproductive roles of seminal plasma melatonin of boar ejaculates used in artificial insemination programs 2017 Access copy available to the general public Unrestricted Melatonin (MLT) is present in seminal plasma (SP) of mammalian species, including pigs, and it is credited with antioxidant properties. This study aims to identify the sources of variation and the role of boar SP MLT on sperm quality and functionality and in vivo fertilizing ability of liquid-stored semen doses used in AI programs. The SP MLT was measured using an ELISA kit in a total of 219 ejaculates collected from 76 boars, and reproductive records of 5,318 AI sows were recorded. Sperm quality was assessed according to motility (computer-aided sperm analysis) and viability (cytometry evaluation). Sperm functionality was assessed according to the cytometric determination of intracellular H2O2 generation, total and mitochondrial O2- production, and lipid peroxidation in liquid AI semen samples stored at 17°C over 144 h. The concentration of SP MLT differed among seasons (P < 0.01) and day length periods (P < 0.001) of the year, demonstrating that the ejaculates collected during the increasing day length period (9.80 ± 1.38 pg/mL, range: 2.75–21.94) had lower SP MLT concentrations than those collected during the decreasing day length period (16.32 ± 1.67 pg/mL, range: 5.02–35.61). The SP MLT also differed (P < 0.001) among boars, among ejaculates within boar, and among portions within the ejaculate, demonstrating that SP from the first 10 mL of sperm-rich ejaculate fraction (SRF) exhibited lower MLT concentrations than post-SRF. The SP MLT was negatively related (P < 0.001) to mitochondrial O2- production in viable sperm. The SP MLT did not differ among AI boars (n = 14) hierarchically grouped according to high and low fertility outcomes. In conclusion, SP MLT concentration in AI boars varies depending on the season of ejaculate collection and differs among boars, ejaculates within boar, and portions within ejaculate. The SP MLT may act at the mitochondrial level of sperm by reducing the generation of O2-. However, this antioxidant role of SP MLT was not reflected in sperm quality or in vivo fertility outcomes of AI semen doses. info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL2013-43328-P info:eu-repo/grantAgreement/ES/MINECO/AGL2015-69738-R info:eu-repo/grantAgreement/ES/MINECO/AGL2012-39903 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 1.711 2017 AGRICULTURE, DAIRY & ANIMAL SCIENCE 12 / 59 = 0.203 2017 Q1 T1 0.848 2017 Animal Science and Zoology 2017 Q1 Food Science 2017 Q1 Medicine (miscellaneous) 2017 Q2 Genetics 2017 Q3 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion (orcid)0000-0003-1997-4262 Casao, A. Universidad de Zaragoza Perez-Patiño, C. Parrilla, I. (orcid)0000-0003-1306-8517 Muiño-Blanco, T. Universidad de Zaragoza Martinez, E.A. (orcid)0000-0001-9842-6197 Cebrian-Perez, J.A. Universidad de Zaragoza Roca, J. 1002 819 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Zoología 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 95, 4 (2017), 1660-1668 J. anim. sci. JOURNAL OF ANIMAL SCIENCE 0021-8812 452570 http://zaguan.unizar.es/record/63224/files/texto_completo.pdf Versión publicada 114987 http://zaguan.unizar.es/record/63224/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:63224 articulos driver 2020-04-28-12:01:18 ARTICLE