63228 20240104111806.0 doi 10.4142/jvs.2017.18.1.39 sideral 98437 ART-2017-98437 eng Barrachina, L. Universidad de Zaragoza (orcid)0000-0001-9818-508X Inflammation affects the viability and plasticity of equine mesenchymal stem cells: Possible implications in intra-articular treatments 2017 Access copy available to the general public Unrestricted Mesenchymal stem cells (MSCs) are gaining relevance for treating equine joint injuries because of their ability to limit inflammation and stimulate regeneration. Because inflammation activates MSC immunoregulatory function, proinflammatory priming could improve MSC efficacy. However, inflammatory molecules present in synovial fluid or added to the culture medium might have deleterious effects on MSCs. Therefore, this study was conducted to investigate the effects of inflammatory synovial fluid and proinflammatory cytokines priming on viability and plasticity of equine MSCs. Equine bone marrow derived MSCs (eBM-MSCs) from three animals were cultured for 72 h in media supplemented with: 20% inflammatory synovial fluid (SF); 50 ng/mL IFN-¿ and TNF-a (CK50); and 20 ng/mL IFN-¿ and TNF-a (CK20). Proliferation assay and expression of proliferation and apoptosis-related genes showed that SF exposed-eBM-MSCs maintained their viability, whereas the viability of CK primed-eBM-MSCs was significantly impaired. Tri-lineage differentiation assay revealed that exposure to inflammatory synovial fluid did not alter eBM-MSCs differentiation potential; however, eBM-MSCs primed with cytokines did not display osteogenic, adipogenic or chondrogenic phenotype. The inflammatory synovial environment is well tolerated by eBM-MSCs, whereas cytokine priming negatively affects the viability and differentiation abilities of eBM-MSCs, which might limit their in vivo efficacy. info:eu-repo/grantAgreement/ES/DGA/LAGENBIO-GROUP info:eu-repo/grantAgreement/ES/MINECO/AGL2011-28609 info:eu-repo/semantics/openAccess by-nc-sa http://creativecommons.org/licenses/by-nc-sa/3.0/es/ 1.327 2017 VETERINARY SCIENCES 48 / 139 = 0.345 2017 Q2 T2 0.469 2017 Veterinary (miscellaneous) 2017 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Remacha, A. R. Universidad de Zaragoza (orcid)0000-0002-1075-8267 Romero, A. Universidad de Zaragoza (orcid)0000-0001-7188-0461 Vázquez, F. J. Universidad de Zaragoza (orcid)0000-0002-8712-2275 Albareda, J. Universidad de Zaragoza (orcid)0000-0003-4489-3130 Prades, M. (orcid)0000-0003-2713-9232 Ranera, B. (orcid)0000-0002-4495-8857 Zaragoza, P. Universidad de Zaragoza (orcid)0000-0001-5740-0185 Martín-Burriel, I. Universidad de Zaragoza (orcid)0000-0001-6016-4726 Rodellar, C. Universidad de Zaragoza (orcid)0000-0003-3289-2675 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1004 830 Universidad de Zaragoza Dpto. Cirugía,Ginecol.Obstetr. Área Traumatología y Ortopedia 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 18, 1 (2017), 39-49 Journal of Veterinary Science Journal of Veterinary Science 1229-845X 6609971 http://zaguan.unizar.es/record/63228/files/texto_completo.pdf Versión publicada 111331 http://zaguan.unizar.es/record/63228/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:63228 articulos driver 2024-01-04-11:00:31 ARTICLE