doi:10.1038/ncomms4025engFratila, Raluca M.Gomez, M. VictoriaSykora, StanislavVelders, Aldrik H.Multinuclear nanoliter one-dimensional and two-dimensional NMR spectroscopy with a single non-resonant microcoilART-2014-85754Nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical technique, but its low sensitivity and highly sophisticated, costly, equipment severely constrain more widespread applications. Here we show that a non-resonant planar transceiver microcoil integrated in a microfluidic chip (detection volume 25¿nl) can detect different nuclides in the full broad-band range of Larmor frequencies (at 9.4¿T from 61 to 400¿MHz). Routine one-dimensional (1D) and two-dimensional (2D), homo- and heteronuclear experiments can be carried out using the broad-band coil set-up. Noteworthy, heteronuclear 2D experiments can be performed in a straightforward manner on virtually any combination of nuclides (from classical 1H–13C to more exotic combinations like 19F–31P) both in coupled and decoupled mode. Importantly, the concept of a non-resonant system provides magnetic field-independent NMR probes; moreover, the small-volume alleviates problems related to field inhomogeneity, making the broad-band coil an attractive option for, for example, portable and table-top NMR systems.2014http://zaguan.unizar.es/record/6327810.1038/ncomms4025http://zaguan.unizar.es/record/63278oai:zaguan.unizar.es:63278NATURE COMMUNICATIONS 5, 3025 (2014), 1-8byhttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccess