Analysis of whole blood by ICP-MS equipped with a high temperature total sample consumption system

Cañabate, Águeda ; García-Ruiz, Esperanza (Universidad de Zaragoza) ; Resano, Martín (Universidad de Zaragoza) ; Todolí, José Luis

Resumen: In this work, the performance of a high temperature torch Integrated Sample Introduction System (hTISIS) using inductively coupled plasma mass spectrometry (ICP-MS) for the multi-element analysis of whole blood is evaluated. Two different quadrupole-based spectrometers were tested and the optimization studies were performed with a reference whole blood material. Sample dilution factor and hTISIS temperature were taken as the variables. The sample was introduced following two different procedures: continuous aspiration and air-segmentation. The optimum performance of the system in terms of both analytical figures of merit and accuracy was obtained for a hTISIS temperature of 200 °C and for either 1 : 10 or 1 : 25 dilution factors depending on the ICP-MS device used. An improvement in terms of sensitivity and detection limits was obtained with the hTISIS as compared to the conventional cyclonic spray chamber. Thus, for 1 : 25 diluted blood samples, the hTISIS improved the sensitivity by a factor ranging 2.0-4.5. Moreover, at 200 °C, the limits of detection for hTISIS at 200 °C were 1.1 to 8.4 times lower than those for the cyclonic spray chamber. This analytical parameter was in the ng L-1 range for the detected elements. Matrix effects in turn became less severe as the hTISIS temperature increased (from room temperature to 80 °C). All these results were obtained without a severe degradation of the fundamental plasma parameters. In fact, under optimum conditions BaO+/Ba+ and Ba2+/Ba+ signal ratios were 1.2% and 2.0%, respectively. The developed method was applied for the analysis of low volumes (ca. 2.5 µL) of real blood samples after a minimally invasive collection by volumetric absorptive microsampling. The hTISIS is an easy-to-implement sample introduction system that can be employed for the routine analysis using ICP-MS.

Idioma: Inglés

DOI: 10.1039/c6ja00374e

Año: 2017

Publicado en: Journal of Analytical Atomic Spectrometry 32, 1 (2017), 78-87 [38 pp.]
ISSN: 0267-9477

Factor impacto JCR: 3.608 (2017)

Categ. JCR: SPECTROSCOPY rank: 4 / 42 = 0.095 (2017) - Q1 - T1
Categ. JCR: CHEMISTRY, ANALYTICAL rank: 13 / 80 = 0.162 (2017) - Q1 - T1

Factor impacto SCIMAGO: 1.066 - Spectroscopy (Q1) - Analytical Chemistry (Q1)

Financiación: info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-64684-P

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)