000063317 001__ 63317
000063317 005__ 20171129112117.0
000063317 0247_ $$2doi$$a10.1371/journal.pone.0066641
000063317 0248_ $$2sideral$$a84288
000063317 037__ $$aART-2013-84288
000063317 041__ $$aeng
000063317 100__ $$aSalces-Ortiz, J.
000063317 245__ $$aOvine HSP90AA1 Expression Rate Is Affected by Several SNPs at the Promoter under Both Basal and Heat Stress Conditions
000063317 260__ $$c2013
000063317 5060_ $$aAccess copy available to the general public$$fUnrestricted
000063317 5203_ $$aThe aim of this work was to investigate the association between polymorphisms located at the HSP90AA1 ovine gene promoter and gene expression rate under different environmental conditions, using a mixed model approach. Blood samples from 120 unrelated rams of the Manchega sheep breed were collected at three time points differing in environmental conditions. Rams were selected on the basis of their genotype for the transversion G/C located 660 base pairs upstream the gene transcription initiation site. Animals were also genotyped for another set of 6 SNPs located at the gene promoter. Two SNPs, G/C-660 and A/G-444, were associated with gene overexpression resulting from heat stress. The composed genotype CC-660-AG-444 was the genotype having the highest expression rates with fold changes ranging from 2.2 to 3.0. The genotype AG-522 showed the highest expression levels under control conditions with a fold change of 1.4. Under these conditions, the composed genotype CC-601-TT-524-AG-522-TT-468 is expected to be correlated with higher basal expression of the gene according to genotype frequencies and linkage disequilibrium values. Some putative transcription factors were predicted for binding sites where the SNPs considered are located. Since the expression rate of the gene under alternative environmental conditions seems to depend on the composed genotype of several SNPs located at its promoter, a cooperative regulation of the transcription of the HSP90AA1 gene could be hypothesized. Nevertheless epigenetic regulation mechanisms cannot be discarded. © 2013 Salces-Ortiz et al.
000063317 536__ $$9info:eu-repo/grantAgreement/ES/INIA/RTA2009-00098-00-00
000063317 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000063317 590__ $$a3.534$$b2013
000063317 591__ $$aMULTIDISCIPLINARY SCIENCES$$b8 / 56 = 0.143$$c2013$$dQ1$$eT1
000063317 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000063317 700__ $$aGonzález, C.
000063317 700__ $$aMoreno-Sánchez, N.
000063317 700__ $$0(orcid)0000-0001-9513-0219$$aCalvo, J.H.
000063317 700__ $$aPérez-Guzmán, M.D.
000063317 700__ $$aSerrano, M.M.
000063317 773__ $$g8, 6 (2013), e66641 [12 pp]$$pPLoS One$$tPLoS One$$x1932-6203
000063317 8564_ $$s500718$$uhttps://zaguan.unizar.es/record/63317/files/texto_completo.pdf$$yVersión publicada
000063317 8564_ $$s126165$$uhttps://zaguan.unizar.es/record/63317/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000063317 909CO $$ooai:zaguan.unizar.es:63317$$particulos$$pdriver
000063317 951__ $$a2017-11-28-13:51:12
000063317 980__ $$aARTICLE