000063327 001__ 63327
000063327 005__ 20171129112117.0
000063327 0247_ $$2doi$$a10.1371/journal.pone.0086107
000063327 0248_ $$2sideral$$a100185
000063327 037__ $$aART-2014-100185
000063327 041__ $$aeng
000063327 100__ $$aRamón, M.
000063327 245__ $$aInfluence of the temperature and the genotype of the HSP90AA1 gene over sperm chromatin stability in manchega rams
000063327 260__ $$c2014
000063327 5060_ $$aAccess copy available to the general public$$fUnrestricted
000063327 5203_ $$aThe present study addresses the effect of heat stress on males'' reproduction ability. For that, we have evaluated the sperm DNA fragmentation (DFI) by SCSA of ejaculates incubated at 37°C during 0, 24 and 48 hours after its collection, as a way to mimic the temperature circumstances to which spermatozoa will be subject to in the ewe uterus. The effects of temperature and temperature-humidity index (THI) from day 60 prior collection to the date of semen collection on DFI were examined. To better understand the causes determining the sensitivity of spermatozoa to heat, this study was conducted in 60 males with alternative genotypes for the SNP G/C-660 of the HSP90AA1 promoter, which encode for the Hsp90a protein. The Hsp90a protein predominates in the brain and testis, and its role in spermatogenesis has been described in several species. Ridge regression analyses showed that days 29 to 35 and 7 to 14 before sperm collection (bsc) were the most critical regarding the effect of heat stress over DFI values. Mixed model analyses revealed that DFI increases over a threshold of 30°C for maximum temperature and 22 for THI at days 29 to 35 and 7 to 14 bsc only in animals carrying the GG-660 genotype. The period 29-35 bsc coincide with the meiosis I process for which the effect of the Hsp90a has been described in mice. The period 7-14 bsc may correspond with later stages of the meiosis II and early stages of epididymal maturation in which the replacement of histones by protamines occurs. Because of GG-660 genotype has been associated to lower levels of HSP90AA1 expression, suboptimal amounts of HSP90AA1 mRNA in GG-660 animals under heat stress conditions make spermatozoa DNA more susceptible to be fragmented. Thus, selecting against the GG-660 genotype could decrease the DNA fragmentation and spermatozoa thermal susceptibility in the heat season, and its putative subsequent fertility gains.
000063327 536__ $$9info:eu-repo/grantAgreement/ES/INIA/RTA2009-00098-00-00
000063327 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000063327 590__ $$a3.234$$b2014
000063327 591__ $$aMULTIDISCIPLINARY SCIENCES$$b9 / 57 = 0.158$$c2014$$dQ1$$eT1
000063327 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000063327 700__ $$aSalces-Ortiz, J.
000063327 700__ $$aGonzález, C.
000063327 700__ $$aPérez-Guzmán, M. D.
000063327 700__ $$aGarde, J. J.
000063327 700__ $$aGarcía-Álvarez, O.
000063327 700__ $$aMaroto-Morales, A.
000063327 700__ $$0(orcid)0000-0001-9513-0219$$aCalvo, J. H.
000063327 700__ $$aSerrano, M. M.
000063327 773__ $$g9, 1 (2014), e86107 [10 pp]$$pPLoS One$$tPLoS One$$x1932-6203
000063327 8564_ $$s270028$$uhttps://zaguan.unizar.es/record/63327/files/texto_completo.pdf$$yVersión publicada
000063327 8564_ $$s130922$$uhttps://zaguan.unizar.es/record/63327/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000063327 909CO $$ooai:zaguan.unizar.es:63327$$particulos$$pdriver
000063327 951__ $$a2017-11-28-13:51:34
000063327 980__ $$aARTICLE