000063674 001__ 63674 000063674 005__ 20171221155149.0 000063674 037__ $$aTAZ-TFM-2017-796 000063674 041__ $$aeng 000063674 1001_ $$aArtigas Ortega, Melania 000063674 24200 $$aCommissioning and equipment assessment of a semi-industrial bioreactor 000063674 24500 $$aCommissioning and equipment assessment of a semi-industrial bioreactor 000063674 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2017 000063674 500__ $$aTrabajo realizado en el departamento PILOT PLANT de la Universidad Técnica de Dinamarca. 000063674 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/ 000063674 520__ $$aTasks regarding the commission and equipment assessment on the fermenter situated in PILOT PLANT Research Center were carried out. The bioreactor consists in a pilotscale fermenter with a capacity of 150L. Piping and Instrumentation Diagrams were elaborated, in which a posterior Sterilization Standard Operating Procedure was designed. In such SOP, Steam In Place method was implemented. The sterilization was designed as a batch process, in which the reaction medium is sterilized simultaneously to the fermentation vessel. This way, the use of additional equipment was avoided and the risk of contamination between sterilization and fermentation start was minimized. Optimal saturated vapor temperature was taken as 121ºC. The required holding time for equipment sterilization (spare parts) was determined to be 10 minutes, whereas a holding time of 20 minutes was considered for medium sterilization. Other key factors taken into account for the design of the procedure were condensate removal, air evacuation and post-sterilization integrity, The SOP is still in need of validation. A sealing test revealed the inoperability of the safety device (burst disk). This device was then replaced and tested with success. Gas-liquid mass transfer capacity of the fermenter was assessed, applying the hydrogen peroxide method. A KLa of 25±3.3 h-1was determined when the stirrer run at 450 rpm. However, due to supersaturation effect, this value could be an overestimation of the actual value. Comparison with other typical KLa values for pilot-scale bioreactors led to the conclusion that the aeration capacity of the fermenter is low and might be insufficient for some biochemical processes. A change in the impeller type is proposed in order to address this problem. 000063674 521__ $$aMáster Universitario en Ingeniería Química 000063674 540__ $$aDerechos regulados por licencia Creative Commons 000063674 700__ $$aLantz, Anna Eliasson$$edir. 000063674 700__ $$aHundebøl, Ivan$$edir. 000063674 7102_ $$aUniversidad de Zaragoza$$b $$c 000063674 7202_ $$aGonzalo Callejo, Alberto Ignacio$$eponente 000063674 8560_ $$f646046@celes.unizar.es 000063674 8564_ $$s13044577$$uhttps://zaguan.unizar.es/record/63674/files/TAZ-TFM-2017-796.pdf$$yMemoria (eng) 000063674 909CO $$ooai:zaguan.unizar.es:63674$$pdriver$$ptrabajos-fin-master 000063674 950__ $$a 000063674 951__ $$adeposita:2017-12-21 000063674 980__ $$aTAZ$$bTFM$$cEINA