000064096 001__ 64096
000064096 005__ 20171221155209.0
000064096 037__ $$aTAZ-TFG-2017-3180
000064096 041__ $$aspa
000064096 1001_ $$aDomínguez Álvarez, Roberto Ignacio
000064096 24200 $$aDevelopment of an optic enzimatic biosensor for the determination of Cholesterol in biological samples
000064096 24500 $$aDesarrollo de un biosensor óptico enzimático para la determinación de Colesterol en muestras biológicas
000064096 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2017
000064096 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/
000064096 520__ $$aThe determination of Cholesterol is very important in the area of clinical analysis. Cholesterol is transported by the blood into the HDL and LDL lipoproteins with free Cholesterol and esterified Cholesterol from the liver to the cells. With high concentrations can lead to cardiovascular diseases because can be added to the arterial walls. The analytical method is through fluorescence during the enzymatic reaction thanks to the characteristics of the active FAD center of Cholesterol Oxidase. Free Cholesterol is soluble in organic solvents and with surfactants in aqueous phase. Testing with all the different solvents, finally was chosen Acetone with mixed phases. Kinetic conditions are performed in the excitation and emission maxims, 450 and 525 nm respectly. The reproducibility of the analytical method has a relative standard deviation of 7.22%. With the chosen analytical method, it was optimized with different pH, concentrations of Cholesterol Oxidase and buffer, choosing as best results. [COD] = 0.76 U/mL with Tris-HCl 50 mM pH= 7 buffer. This method can be used in the future for the development of the biosensor.
000064096 521__ $$aGraduado en Química
000064096 540__ $$aDerechos regulados por licencia Creative Commons
000064096 700__ $$aGalbán Bernal, Francisco Javier$$edir.
000064096 7102_ $$aUniversidad de Zaragoza$$bQuímica Analítica$$cQuímica Analítica
000064096 8560_ $$f499010@celes.unizar.es
000064096 8564_ $$s1928201$$uhttps://zaguan.unizar.es/record/64096/files/TAZ-TFG-2017-3180.pdf$$yMemoria (spa)
000064096 8564_ $$s486936$$uhttps://zaguan.unizar.es/record/64096/files/TAZ-TFG-2017-3180_ANE.pdf$$yAnexos (spa)
000064096 909CO $$ooai:zaguan.unizar.es:64096$$pdriver$$ptrabajos-fin-grado
000064096 950__ $$a
000064096 951__ $$adeposita:2017-12-21
000064096 980__ $$aTAZ$$bTFG$$cCIEN