64496 20190709135527.0 doi 10.1002/cpt.639 sideral 97965 ART-2017-97965 eng Patrignani, P Low-dose aspirin acetylates cyclooxygenase-1 in human colorectal mucosa: implications for the chemoprevention of colorectal cancer. 2017 Access copy available to the general public Unrestricted The mechanism of action of low-dose aspirin in the prevention of colorectal cancer (CRC) remains largely hypothetical. We aimed to compare the effects of low-dose aspirin (100 mg/day for 7 days) given to 40 individuals undergoing CRC screening on the extent of cyclooxygenase (COX)-1 acetylation at serine-529 (AceCOX-1), in blood platelets vs colorectal mucosa, at 7 (group 1) and 24 hours (group 2) after dosing. A significantly (P<0.01) lower %AceCOX-1 was detected in colonic and rectal mucosa (average 64%) vs platelets (average 75%) in both groups. This effect was associated with an average 46% (P<0.01) and 35% (P<0.05) reduction in prostaglandin (PG) E2 levels and phosphorylated S6 (p-S6) levels, respectively. Rectal mucosal levels of p-S6/S6 significantly(P<0.01) correlated with PGE2. These findings demonstrate that low-dose aspirin produces long-lasting acetylation of COX-1 and down-regulation of p-S6 in human colorectal mucosa, an effect that may interfere with early colorectal carcinogenesis. info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 6.544 2017 PHARMACOLOGY & PHARMACY 13 / 261 = 0.05 2017 Q1 T1 1.699 2017 Pharmacology (medical) 2017 Q1 Pharmacology 2017 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Sacco, A Sostres, C Bruno, A Dovizio, M (orcid)0000-0001-5813-3445 Piazuelo, E Universidad de Zaragoza Di Francesco, L Contursi, A Zucchelli, M Schiavone, S Tacconelli, S Patrono, C (orcid)0000-0001-5932-2889 Lanas, A. Universidad de Zaragoza 1005 410 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Fisiología 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 36 (2017), [29 pp.] Clin. pharmacol. ther. CLINICAL PHARMACOLOGY & THERAPEUTICS 0009-9236 862975 http://zaguan.unizar.es/record/64496/files/texto_completo.pdf Postprint 116758 http://zaguan.unizar.es/record/64496/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:64496 articulos driver 2019-07-09-12:00:16 ARTICLE