000064497 001__ 64497
000064497 005__ 20190709135530.0
000064497 0247_ $$2doi$$a10.1016/j.jcpa.2016.11.073
000064497 0248_ $$2sideral$$a98333
000064497 037__ $$aART-2017-98333
000064497 041__ $$aeng
000064497 100__ $$aGimeno, M.
000064497 245__ $$aBiocompatibility Studies of Local Antibiotic-eluting Devices for Orthopaedic Applications
000064497 260__ $$c2017
000064497 5060_ $$aAccess copy available to the general public$$fUnrestricted
000064497 5203_ $$aIntroduction: Efficient local antibiotic-eluting devices could be an alternative for delivery of locally therapeutic antibiotics into tissues, avoiding bacterial contamination on implanted materials and minimizing side effects. A proper assessment of biocompatibility of the biomaterials used is important to improve safety after implantation. We present cytotoxicological and implantation tests results to evaluate biocompatibility of two drug-eluting systems with potential use in orthopaedic implants.
Materials and Methods: Cytotoxicological studies were carried out by evaluating the in-vitro dose-dependent effect of cefazolin and linezolid on fibroblasts, keratinocytes, macrophages and osteoblasts. Cells were incubated with antibiotic concentrations ranging from 0.25 to 1.5 mg/ml. Cellular viability was assessed by the Alamar blue test. Cell cycle and apoptosis were measured by flow cytometry. Short-term implantation tests were performed in an ovine model to assess the local effects of the device. Two implants were used: (1) a macroporous stainless steel reservoir loaded with linezolid and (2) stainless steel pins with orifices drilled in the reservoir wall loaded with cefazolin. Implants were placed in sheep tibias. Tissues were studied by pathological means, determining the local effect and tissue response from the implant. (Ethical committee approval number: PI36/14)
Results: Cytotoxic effects of cefazolin and linezolid were only found at 1.5 mg/ml on keratinocytes and osteoblasts, respectively. There were no significant changes on cell cycle and apoptosis at 1.0 mg/ml. Sheep with both antibiotic-loaded implants did not show local or systemic adverse effects.
Conclusions: These results showed no potential toxic effects for the designed devices. However, the antibiotic local concentration should not exceed 1.0 mg/ml.
000064497 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000064497 590__ $$a1.364$$b2017
000064497 591__ $$aVETERINARY SCIENCES$$b46 / 140 = 0.329$$c2017$$dQ2$$eT1
000064497 591__ $$aPATHOLOGY$$b58 / 79 = 0.734$$c2017$$dQ3$$eT3
000064497 592__ $$a0.594$$b2017
000064497 593__ $$aVeterinary (miscellaneous)$$c2017$$dQ2
000064497 593__ $$aPathology and Forensic Medicine$$c2017$$dQ2
000064497 655_4 $$ainfo:eu-repo/semantics/conferenceObject$$vinfo:eu-repo/semantics/acceptedVersion
000064497 700__ $$0(orcid)0000-0002-9210-6026$$aPinczowski, P.
000064497 700__ $$0(orcid)0000-0003-2293-363X$$aMendoza, G.
000064497 700__ $$0(orcid)0000-0002-8712-2275$$aVázquez, F.J.$$uUniversidad de Zaragoza
000064497 700__ $$0(orcid)0000-0002-8133-2124$$aPérez, M.$$uUniversidad de Zaragoza
000064497 700__ $$0(orcid)0000-0002-6178-4801$$aAsín, J.$$uUniversidad de Zaragoza
000064497 700__ $$0(orcid)0000-0002-8701-9745$$aSantamaría, J.$$uUniversidad de Zaragoza
000064497 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, M.$$uUniversidad de Zaragoza
000064497 700__ $$0(orcid)0000-0002-2053-9842$$aLuján, L.$$uUniversidad de Zaragoza
000064497 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000064497 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000064497 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000064497 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000064497 773__ $$g156, 1 (2017), 77$$pJ. Comp. Pathol.$$tJOURNAL OF COMPARATIVE PATHOLOGY$$x0021-9975
000064497 8564_ $$s19642$$uhttps://zaguan.unizar.es/record/64497/files/texto_completo.pdf$$yPostprint
000064497 8564_ $$s92731$$uhttps://zaguan.unizar.es/record/64497/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000064497 909CO $$ooai:zaguan.unizar.es:64497$$particulos$$pdriver
000064497 951__ $$a2019-07-09-12:01:49
000064497 980__ $$aARTICLE