64497 20190709135530.0 doi 10.1016/j.jcpa.2016.11.073 sideral 98333 ART-2017-98333 eng Gimeno, M. Biocompatibility Studies of Local Antibiotic-eluting Devices for Orthopaedic Applications 2017 Access copy available to the general public Unrestricted Introduction: Efficient local antibiotic-eluting devices could be an alternative for delivery of locally therapeutic antibiotics into tissues, avoiding bacterial contamination on implanted materials and minimizing side effects. A proper assessment of biocompatibility of the biomaterials used is important to improve safety after implantation. We present cytotoxicological and implantation tests results to evaluate biocompatibility of two drug-eluting systems with potential use in orthopaedic implants. Materials and Methods: Cytotoxicological studies were carried out by evaluating the in-vitro dose-dependent effect of cefazolin and linezolid on fibroblasts, keratinocytes, macrophages and osteoblasts. Cells were incubated with antibiotic concentrations ranging from 0.25 to 1.5 mg/ml. Cellular viability was assessed by the Alamar blue test. Cell cycle and apoptosis were measured by flow cytometry. Short-term implantation tests were performed in an ovine model to assess the local effects of the device. Two implants were used: (1) a macroporous stainless steel reservoir loaded with linezolid and (2) stainless steel pins with orifices drilled in the reservoir wall loaded with cefazolin. Implants were placed in sheep tibias. Tissues were studied by pathological means, determining the local effect and tissue response from the implant. (Ethical committee approval number: PI36/14) Results: Cytotoxic effects of cefazolin and linezolid were only found at 1.5 mg/ml on keratinocytes and osteoblasts, respectively. There were no significant changes on cell cycle and apoptosis at 1.0 mg/ml. Sheep with both antibiotic-loaded implants did not show local or systemic adverse effects. Conclusions: These results showed no potential toxic effects for the designed devices. However, the antibiotic local concentration should not exceed 1.0 mg/ml. info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 1.364 2017 VETERINARY SCIENCES 46 / 140 = 0.329 2017 Q2 T1 PATHOLOGY 58 / 79 = 0.734 2017 Q3 T3 0.594 2017 Veterinary (miscellaneous) 2017 Q2 Pathology and Forensic Medicine 2017 Q2 info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/acceptedVersion (orcid)0000-0002-9210-6026 Pinczowski, P. (orcid)0000-0003-2293-363X Mendoza, G. (orcid)0000-0002-8712-2275 Vázquez, F.J. Universidad de Zaragoza (orcid)0000-0002-8133-2124 Pérez, M. Universidad de Zaragoza (orcid)0000-0002-6178-4801 Asín, J. Universidad de Zaragoza (orcid)0000-0002-8701-9745 Santamaría, J. Universidad de Zaragoza (orcid)0000-0003-3165-0156 Arruebo, M. Universidad de Zaragoza (orcid)0000-0002-2053-9842 Luján, L. Universidad de Zaragoza 1001 025 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Anatom.Anatom.Patológ.Com 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 156, 1 (2017), 77 J. Comp. Pathol. JOURNAL OF COMPARATIVE PATHOLOGY 0021-9975 19642 http://zaguan.unizar.es/record/64497/files/texto_completo.pdf Postprint 92731 http://zaguan.unizar.es/record/64497/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:64497 articulos driver 2019-07-09-12:01:49 ARTICLE