64502 20210121114542.0 doi 10.3852/15-027 sideral 103933 ART-2015-103933 eng (orcid)0000-0003-4076-6118 Ferreira, Patricia Universidad de Zaragoza A survey of genes encoding H2O2-producing GMC oxidoreductases in 10 Polyporales genomes 2015 Access copy available to the general public Unrestricted The genomes of three representative Polyporales (Bjerkandera adusta, Phlebia brevispora and a member of the Ganoderma lucidum complex) recently were sequenced to expand our knowledge on the diversity and distribution of genes involved in degradation of plant polymers in this Basidiomycota order, which includes most wood-rotting fungi. Oxidases, including members of the glucose-methanol-choline (GMC) oxidoreductase superfamily, play a central role in the above degradative process because they generate extracellular H2O2 acting as the ultimate oxidizer in both white-rot and brown-rot decay. The survey was completed by analyzing the GMC genes in the available genomes of seven more species to cover the four Polyporales clades. First, an in silico search for sequences encoding members of the aryl-alcohol oxidase, glucose oxidase, methanol oxidase, pyranose oxidase, cellobiose dehydrogenase and pyranose dehydrogenase families was performed. The curated sequences were subjected to an analysis of their evolutionary relationships, followed by estimation of gene duplication/ reduction history during fungal evolution. Second, the molecular structures of the near one hundred GMC oxidoreductases identified were modeled to gain insight into their structural variation and expected catalytic properties. In contrast to ligninolytic peroxidases, whose genes are present in all white-rot Polyporales genomes and absent from those of brown-rot species, the H2O2-generating oxidases are widely distributed in both fungal types. This indicates that the GMC oxidases provide H2O2 for both ligninolytic peroxidase activity (in white-rot decay) and Fenton attack on cellulose (in brown-rot decay), after the transition between both decay patterns in Polyporales occurred. info:eu-repo/grantAgreement/ES/MICINN/BIO2011-26694 info:eu-repo/grantAgreement/EC/FP7/613549/EU/Optimized oxidoreductases for medium and large scale industrial biotransformations/INDOX info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 2.638 2015 MYCOLOGY 9 / 29 = 0.31 2015 Q2 T1 1.133 2015 Medicine (miscellaneous) 2015 Q1 Plant Science 2015 Q1 Ecology, Evolution, Behavior and Systematics 2015 Q1 Physiology 2015 Q2 Genetics 2015 Q2 Molecular Biology 2015 Q2 Cell Biology 2015 Q3 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Carro, J. Serrano, A. Martinez, A.T. 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 107, 6 (2015), 1105-1119 Mycologia MYCOLOGIA 0027-5514 3515038 http://zaguan.unizar.es/record/64502/files/texto_completo.pdf Versión publicada 123040 http://zaguan.unizar.es/record/64502/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:64502 articulos driver 2021-01-21-11:16:47 ARTICLE