doi:10.3389/fimmu.2017.01817engNúñez, D.Comas, L.Lanuza, P.M.Sánchez-Martinez, D.Pérez-Hernández, M.Catalán, E.Domingo, M.P.Velázquez-Campoy, A.Pardo, J.Gálvez, E.M.A functional analysis on the interspecies interaction between mouse LFA-1 and human intercellular adhesion molecule-1 at the cell levelART-2017-104253The interaction between intercellular adhesion molecules (ICAM) and the integrin leukocyte function-associated antigen-1 (LFA-1) is crucial for the regulation of several physiological and pathophysiological processes like cell-mediated elimination of tumor or virus infected cells, cancer metastasis, or inflammatory and autoimmune processes. Using purified proteins it was reported a species restriction for the interaction of ICAM-1 and LFA-1, being mouse ICAM-1 able to interact with human LFA-1 but not human ICAM-1 with mouse LFA-1. However, in vivo results employing tumor cells transfected with human ICAM-1 suggest that functionally mouse LFA-1 can recognize human ICAM-1. In order to clarify the interspecies cross-reactivity of the ICAM-1/LFA-1 interaction, we have performed functional studies analyzing the ability of human soluble ICAM-1 and human/mouse LFA-1 derived peptides to inhibit cell aggregation and adhesion as well as cell-mediated cytotoxicity in both mouse and human systems. In parallel, the affinity of the interaction between mouse LFA-1-derived peptides and human ICAM-1 was determined by calorimetry assays. According to the results obtained, it seems that human ICAM-1 is able to interact with mouse LFA-1 on intact cells, which should be taking into account when using humanized mice and xenograft models for the study of immune-related processes.2017http://zaguan.unizar.es/record/6532110.3389/fimmu.2017.01817http://zaguan.unizar.es/record/65321oai:zaguan.unizar.es:65321info:eu-repo/grantAgreement/ES/DGA/FSEinfo:eu-repo/grantAgreement/ES/MINECO/BFU2016-78232-Pinfo:eu-repo/grantAgreement/ES/MINECO-FEDER/SAF2011-25390info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-1-Rinfo:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-2-RFrontiers in Immunology 8, DEC (2017), 817 [12 pp]byhttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccess