65321 20190709135643.0 doi 10.3389/fimmu.2017.01817 sideral 104253 ART-2017-104253 eng Núñez, D. A functional analysis on the interspecies interaction between mouse LFA-1 and human intercellular adhesion molecule-1 at the cell level 2017 Access copy available to the general public Unrestricted The interaction between intercellular adhesion molecules (ICAM) and the integrin leukocyte function-associated antigen-1 (LFA-1) is crucial for the regulation of several physiological and pathophysiological processes like cell-mediated elimination of tumor or virus infected cells, cancer metastasis, or inflammatory and autoimmune processes. Using purified proteins it was reported a species restriction for the interaction of ICAM-1 and LFA-1, being mouse ICAM-1 able to interact with human LFA-1 but not human ICAM-1 with mouse LFA-1. However, in vivo results employing tumor cells transfected with human ICAM-1 suggest that functionally mouse LFA-1 can recognize human ICAM-1. In order to clarify the interspecies cross-reactivity of the ICAM-1/LFA-1 interaction, we have performed functional studies analyzing the ability of human soluble ICAM-1 and human/mouse LFA-1 derived peptides to inhibit cell aggregation and adhesion as well as cell-mediated cytotoxicity in both mouse and human systems. In parallel, the affinity of the interaction between mouse LFA-1-derived peptides and human ICAM-1 was determined by calorimetry assays. According to the results obtained, it seems that human ICAM-1 is able to interact with mouse LFA-1 on intact cells, which should be taking into account when using humanized mice and xenograft models for the study of immune-related processes. info:eu-repo/grantAgreement/ES/DGA/FSE info:eu-repo/grantAgreement/ES/MINECO/BFU2016-78232-P info:eu-repo/grantAgreement/ES/MINECO-FEDER/SAF2011-25390 info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-1-R info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-2-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.511 2017 IMMUNOLOGY 30 / 155 = 0.194 2017 Q1 T1 2.803 2017 Immunology and Allergy 2017 Q1 Immunology 2017 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Comas, L. (orcid)0000-0001-7328-2094 Lanuza, P.M. Universidad de Zaragoza Sánchez-Martinez, D. Pérez-Hernández, M. Catalán, E. Domingo, M.P. (orcid)0000-0001-5702-4538 Velázquez-Campoy, A. Universidad de Zaragoza (orcid)0000-0003-0154-0730 Pardo, J. Universidad de Zaragoza Gálvez, E.M. 1008 566 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Inmunología 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 8, DEC (2017), 817 [12 pp] Front. immunol. Frontiers in Immunology 1664-3224 334547 http://zaguan.unizar.es/record/65321/files/texto_completo.pdf Versión publicada 102368 http://zaguan.unizar.es/record/65321/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:65321 articulos driver 2019-07-09-12:39:14 ARTICLE