65620 20190709135447.0 doi 10.1016/j.actbio.2017.01.001 sideral 98164 ART-2017-98164 eng Kuchler-Bopp, S. Promoting bioengineered tooth innervation using nanostructured and hybrid scaffolds 2017 Access copy available to the general public Unrestricted The innervation of teeth mediated by axons originating from the trigeminal ganglia is essential for their function and protection. Immunosuppressive therapy using Cyclosporine A (CsA) was found to accelerate the innervation of transplanted tissues and particularly that of bioengineered teeth. To avoid the CsA side effects, we report in this study the preparation of CsA loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles, their embedding on polycaprolactone (PCL)-based scaffolds and their possible use as templates for the innervation of bioengineered teeth. This PCL scaffold, approved by the FDA and capable of mimicking the extracellular matrix, was obtained by electrospinning and decorated with CsA-loaded PLGA nanoparticles to allow a local sustained action of this immunosuppressive drug. Dental re-associations were co-implanted with a trigeminal ganglion on functionalized scaffolds containing PLGA and PLGA/cyclosporine in adult ICR mice during 2 weeks. Histological analyses showed that the designed scaffolds did not alter the teeth development after in vivo implantation. The study of the innervation of the dental re-associations by indirect immunofluorescence and transmission electron microscopy (TEM), showed that 88.4% of the regenerated teeth were innervated when using the CsA-loaded PLGA scaffold. The development of active implants thus allows their potential use in the context of dental engineering. Statement of Significance Tooth innervation is essential for their function and protection and this can be promoted in vivo using polymeric scaffolds functionalized with immunosuppressive drug-loaded nanoparticles. Immunosuppressive therapy using biodegradable nanoparticles loaded with Cyclosporine A was found to accelerate the innervation of bioengineered teeth after two weeks of implantation. info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 6.383 2017 MATERIALS SCIENCE, BIOMATERIALS 3 / 33 = 0.091 2017 Q1 T1 ENGINEERING, BIOMEDICAL 4 / 78 = 0.051 2017 Q1 T1 1.967 2017 Biochemistry 2017 Q1 Biomaterials 2017 Q1 Biomedical Engineering 2017 Q1 Biotechnology 2017 Q1 Medicine (miscellaneous) 2017 Q1 Molecular Biology 2017 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion (orcid)0000-0002-6277-7996 Larrea, A. Petry, L. Idoux-Gillet, Y. (orcid)0000-0002-6873-5244 Sebastian, V. Universidad de Zaragoza Ferrandon, A. Schwinté, P. (orcid)0000-0003-3165-0156 Arruebo, M. Universidad de Zaragoza Benkirane-Jessel, N. 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 50 (2017), 493-501 Acta Biomater. ACTA BIOMATERIALIA 1742-7061 1344542 http://zaguan.unizar.es/record/65620/files/texto_completo.pdf Postprint 55467 http://zaguan.unizar.es/record/65620/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:65620 articulos driver 2019-07-09-11:38:41 ARTICLE