000069163 001__ 69163
000069163 005__ 20180228135116.0
000069163 0247_ $$2doi$$a10.1128/AEM.70.2.1249-1251.2004
000069163 0248_ $$2sideral$$a86285
000069163 037__ $$aART-2004-86285
000069163 041__ $$aeng
000069163 100__ $$0(orcid)0000-0001-5664-1729$$aAbian,O.
000069163 245__ $$aStabilization of Penicillin G Acylase from Escherichia coli: Site-Directed Mutagenesis of the Protein Surface to Increase Multipoint Covalent Attachment
000069163 260__ $$c2004
000069163 5060_ $$aAccess copy available to the general public$$fUnrestricted
000069163 5203_ $$aThree mutations on the penicillin acylase surface (increasing the number of Lys in a defined area) were performed. They did not alter the enzyme's stability and kinetic properties; however, after immobilization on glyoxyl-agarose, the mutant enzyme showed improved stability under all tested conditions (e.g., pH 2.5 at 4°C, pH 5 at 60°C, pH 7 at 55°C, or 60% dimethylformamide), with stabilization factors ranging from 4 to 11 compared with the native enzyme immobilized on glyoxyl-agarose.
000069163 536__ $$9info:eu-repo/grantAgreement/ES/CICYT/BIO2000-0747-C05-02$$9info:eu-repo/grantAgreement/ES/CICYT/BIO2001-2259
000069163 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000069163 590__ $$a3.81$$b2004
000069163 591__ $$aMICROBIOLOGY$$b17 / 84 = 0.202$$c2004$$dQ1$$eT1
000069163 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b16 / 131 = 0.122$$c2004$$dQ1$$eT1
000069163 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000069163 700__ $$aGrazú,V.
000069163 700__ $$aHermoso,J.
000069163 700__ $$aGonzález,R.
000069163 700__ $$aGarcía,J. L.
000069163 700__ $$aFernández-Lafuente,R.
000069163 700__ $$aGuisán,J. M.
000069163 773__ $$g70, 2 (2004), 1249-1251$$pAppl. environ. microbiol.$$tAPPLIED AND ENVIRONMENTAL MICROBIOLOGY$$x0099-2240
000069163 8564_ $$s62141$$uhttps://zaguan.unizar.es/record/69163/files/texto_completo.pdf$$yVersión publicada
000069163 8564_ $$s136400$$uhttps://zaguan.unizar.es/record/69163/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000069163 909CO $$ooai:zaguan.unizar.es:69163$$pdriver$$particulos
000069163 951__ $$a2018-02-28-10:22:56
000069163 980__ $$aARTICLE