69712 20190709135611.0 doi 10.1039/c7ra08359a sideral 104733 ART-2017-104733 eng Regiel-Futyra, A. Development of noncytotoxic silver-chitosan nanocomposites for efficient control of biofilm forming microbes 2017 Access copy available to the general public Unrestricted Severe bacterial and fungal infections have become a major clinical and public health concern. Nowadays, additional efforts are needed to develop effective antimicrobial materials that are not harmful to human cells. This work describes the synthesis and characterization of chitosan-ascorbic acid-silver nanocomposites as films exhibiting high antimicrobial activity and non-cytotoxicity towards human cells. The reductive and stabilizing activity of both the biocompatible polymer chitosan and ascorbic acid were used in the synthesis of silver nanoparticles (AgNPs). Herein, we propose an improved composite synthesis based on medium average molecular weight chitosan with a high deacetylation degree, that together with ascorbic acid gave films with a uniform distribution of small AgNPs (<10 nm) exhibiting high antimicrobial activity against biofilm forming bacterial and fungal strains of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans. At the same time, the resulting solid nanocomposites showed, at the same doses, reduced or totally excluded cytotoxicity on mammalian somatic and tumoral cells. Data obtained in the present study suggest that adequately designed chitosan-silver nanocomposites are powerful and promising materials for reducing pathogenic microorganism-associated infections without harmful effects towards mammalian cells. info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM info:eu-repo/semantics/openAccess by-nc http://creativecommons.org/licenses/by-nc/3.0/es/ 2.936 2017 CHEMISTRY, MULTIDISCIPLINARY 71 / 171 = 0.415 2017 Q2 T2 0.863 2017 Chemistry (miscellaneous) 2017 Q1 Chemical Engineering (miscellaneous) 2017 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Kus-Liskiewicz, M. (orcid)0000-0002-6873-5244 Sebastian, V. Universidad de Zaragoza (orcid)0000-0002-2966-9088 Irusta, S. Universidad de Zaragoza (orcid)0000-0003-3165-0156 Arruebo, M. Universidad de Zaragoza Kyziol, A. Stochel, G. 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 7, 83 (2017), 52398-52413 RSC ADVANCES RSC Advances 2046-2069 737689 http://zaguan.unizar.es/record/69712/files/texto_completo.pdf Versión publicada 117267 http://zaguan.unizar.es/record/69712/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:69712 articulos driver 2019-07-09-12:22:42 ARTICLE