69929 20200716101419.0 doi 10.1177/1464420717704880 sideral 104429 ART-2019-104429 eng (orcid)0000-0003-2770-4617 Ortilles, A. Use of 2% hydroxypropyl methylcellulose to prevent the corneal swelling during the in vitro mechanical characterization 2019 Access copy available to the general public Unrestricted The aim of this study was to assess the use of 2% HPMC during in vitro uniaxial tensile tests, with corneal strips immediately obtained or after storing the eyes for 24¿h in 0.9% NaCl solution at 4¿¿. The purpose was to establish a standardized procedure to prevent phenomena which can modify the mechanical properties of the tissue. Rabbit eyes were divided into four groups. Group A had seven eyes that were preserved in NaCl solution for 24¿h before testing. Group B had seven eyes that were immediately tested. In both groups, to prevent both swelling and dehydration, 2% hydroxypropyl methylcellulose (2% HPMC) was applied. Group C had seven eyes that were preserved in NaCl solution for 24¿h before testing. Group D had seven eyes that were immediately tested. In both groups, HPMC was not applied. Regarding the mechanical response, groups with HPMC showed similar Cauchy stress–stretch curves and there were no statistically significant differences at 5%, 10% and 15% strain between them, which mean that both showed similar mechanical behavior. The same result was obtained between groups without HPMC. However, for coupled groups with and without HPMC, statistically significant differences at 10% and 15% strain were observed. On the other hand, when grouped by storage time, statistically significant differences were found between groups that had eyes preserved for 24¿h with and without HPMC, respectively, as well as between groups immediately tested with and without HPMC, respectively, at 15% strain. Nevertheless, if coupled groups were considered, between groups that were preserved for 24¿h in NaCl before testing and groups that were immediately tested, no statistically significant differences were obtained. In addition, the Cauchy stress–stretch curves of groups without HPMC showed a decreasing slope of the linear part (strain¿>¿8%) of the graph during the experiment. In summary, the use of HPMC during the handling of the tissue from excision to testing seems to prevent both swelling and dehydration. info:eu-repo/grantAgreement/ES/MINECO/DPI2014-54981-R info:eu-repo/grantAgreement/ES/MEC/FPU13-03782 info:eu-repo/grantAgreement/EC/FP7/606634/EU/Development of corneal biomechanical model. Dynamic topographical characterization based on 3D plenoptic imaging/POPCORN info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 2.014 2019 MATERIALS SCIENCE, MULTIDISCIPLINARY 193 / 314 = 0.615 2019 Q3 T2 0.376 2019 Mechanical Engineering 2019 Q2 Materials Science (miscellaneous) 2019 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion (orcid)0000-0002-2959-0628 Lanchares, E. Universidad de Zaragoza (orcid)0000-0002-4295-8335 Cristóbal, J.A. Universidad de Zaragoza (orcid)0000-0001-9713-1813 Calvo, B. Universidad de Zaragoza 2007 265 Universidad de Zaragoza Dpto. Métodos Estadísticos Área Estadís. Investig. Opera. 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 233, 5 (2019), 104429 [8 pp.] Proc. Inst. Mech. Eng., Part L, J. Mat. PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART L-JOURNAL OF MATERIALS-DESIGN AND APPLICATIONS 1464-4207 588780 http://zaguan.unizar.es/record/69929/files/texto_completo.pdf Postprint 25854 http://zaguan.unizar.es/record/69929/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:69929 articulos driver 2020-07-16-08:38:56 ARTICLE