000069935 001__ 69935
000069935 005__ 20240122154813.0
000069935 0247_ $$2doi$$a10.1177/2050640617727180
000069935 0248_ $$2sideral$$a102262
000069935 037__ $$aART-2018-102262
000069935 041__ $$aeng
000069935 100__ $$0(orcid)0000-0002-5797-3909$$aLatorre, E.$$uUniversidad de Zaragoza
000069935 245__ $$aToll-like receptors 2 and 4 modulate intestinal IL-10 differently in ileum and colon
000069935 260__ $$c2018
000069935 5060_ $$aAccess copy available to the general public$$fUnrestricted
000069935 5203_ $$aBackground: Inflammatory bowel diseases are consequence of an intestinal homeostasis breakdown in which innate immune dysregulation is implicated. Toll-like receptor (TLR)2 and TLR4 are immune recognition receptors expressed in the intestinal epithelium, the first physical-physiological barrier for microorganisms, to inform the host of the presence of Gram-positive and Gram-negative organisms. Interleukin (IL)-10 is an essential anti-inflammatory cytokine that contributes to maintenance of intestinal homeostasis. Aim: Our main aim was to investigate intestinal IL-10 synthesis and release, and whether TLR2 and TLR4 are determinants of IL-10 expression in the intestinal tract. Methods: We used Caco-2 cell line as an enterocyte-like cell model, and also ileum and colon from mice deficient in TLR2, TLR4 or TLR2/4 to test the involvement of TLR signaling. Results: Intestinal epithelial cells are able to synthesize and release IL-10 and their expression is increased after TLR2 or TLR4 activation. IL-10 regulation seems to be tissue specific, with IL-10 expression in the ileum regulated by a compensation between TLR2 and TLR4 expression, whereas in the colon, TLR2 and TLR4 affect IL-10 expression independently. Conclusions: Intestinal epithelial cells could release IL-10 in response to TLR activation, playing an intestinal tissue-dependent and critical intestinal immune role.
000069935 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B61$$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/BFU2010-18971$$9info:eu-repo/grantAgreement/ES/UZ/2014-BIO-03
000069935 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000069935 590__ $$a3.453$$b2018
000069935 591__ $$aGASTROENTEROLOGY & HEPATOLOGY$$b33 / 84 = 0.393$$c2018$$dQ2$$eT2
000069935 592__ $$a1.329$$b2018
000069935 593__ $$aOncology$$c2018$$dQ1
000069935 593__ $$aGastroenterology$$c2018$$dQ1
000069935 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000069935 700__ $$0(orcid)0000-0001-5573-6144$$aLayunta, E.
000069935 700__ $$0(orcid)0000-0002-5306-9365$$aGrasa, L.$$uUniversidad de Zaragoza
000069935 700__ $$0(orcid)0000-0003-0154-0730$$aPardo, J.$$uUniversidad de Zaragoza
000069935 700__ $$0(orcid)0000-0003-3970-5457$$aGarcía, S.$$uUniversidad de Zaragoza
000069935 700__ $$0(orcid)0000-0002-9935-927X$$aAlcalde, A. I.
000069935 700__ $$0(orcid)0000-0003-4758-3998$$aMesonero, J. E.$$uUniversidad de Zaragoza
000069935 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología
000069935 7102_ $$11008$$2566$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Inmunología
000069935 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina
000069935 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular
000069935 773__ $$g6, 3 (2018), 446-453$$pUnited European Gastroenterol. j.$$tUnited European Gastroenterology Journal$$x2050-6406
000069935 8564_ $$s422786$$uhttps://zaguan.unizar.es/record/69935/files/texto_completo.pdf$$yVersión publicada
000069935 8564_ $$s91550$$uhttps://zaguan.unizar.es/record/69935/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000069935 909CO $$ooai:zaguan.unizar.es:69935$$particulos$$pdriver
000069935 951__ $$a2024-01-22-15:32:04
000069935 980__ $$aARTICLE