69935 20240122154813.0 doi 10.1177/2050640617727180 sideral 102262 ART-2018-102262 eng Latorre, E. Universidad de Zaragoza (orcid)0000-0002-5797-3909 Toll-like receptors 2 and 4 modulate intestinal IL-10 differently in ileum and colon 2018 Access copy available to the general public Unrestricted Background: Inflammatory bowel diseases are consequence of an intestinal homeostasis breakdown in which innate immune dysregulation is implicated. Toll-like receptor (TLR)2 and TLR4 are immune recognition receptors expressed in the intestinal epithelium, the first physical-physiological barrier for microorganisms, to inform the host of the presence of Gram-positive and Gram-negative organisms. Interleukin (IL)-10 is an essential anti-inflammatory cytokine that contributes to maintenance of intestinal homeostasis. Aim: Our main aim was to investigate intestinal IL-10 synthesis and release, and whether TLR2 and TLR4 are determinants of IL-10 expression in the intestinal tract. Methods: We used Caco-2 cell line as an enterocyte-like cell model, and also ileum and colon from mice deficient in TLR2, TLR4 or TLR2/4 to test the involvement of TLR signaling. Results: Intestinal epithelial cells are able to synthesize and release IL-10 and their expression is increased after TLR2 or TLR4 activation. IL-10 regulation seems to be tissue specific, with IL-10 expression in the ileum regulated by a compensation between TLR2 and TLR4 expression, whereas in the colon, TLR2 and TLR4 affect IL-10 expression independently. Conclusions: Intestinal epithelial cells could release IL-10 in response to TLR activation, playing an intestinal tissue-dependent and critical intestinal immune role. info:eu-repo/grantAgreement/ES/DGA/B61 info:eu-repo/grantAgreement/ES/MICINN-FEDER/BFU2010-18971 info:eu-repo/grantAgreement/ES/UZ/2014-BIO-03 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 3.453 2018 GASTROENTEROLOGY & HEPATOLOGY 33 / 84 = 0.393 2018 Q2 T2 1.329 2018 Oncology 2018 Q1 Gastroenterology 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Layunta, E. (orcid)0000-0001-5573-6144 Grasa, L. Universidad de Zaragoza (orcid)0000-0002-5306-9365 Pardo, J. Universidad de Zaragoza (orcid)0000-0003-0154-0730 García, S. Universidad de Zaragoza (orcid)0000-0003-3970-5457 Alcalde, A. I. (orcid)0000-0002-9935-927X Mesonero, J. E. Universidad de Zaragoza (orcid)0000-0003-4758-3998 1005 410 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Fisiología 1008 566 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Inmunología 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 1002 050 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Biología Celular 6, 3 (2018), 446-453 United European Gastroenterol. j. United European Gastroenterology Journal 2050-6406 422786 http://zaguan.unizar.es/record/69935/files/texto_completo.pdf Versión publicada 91550 http://zaguan.unizar.es/record/69935/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:69935 articulos driver 2024-01-22-15:32:04 ARTICLE