70053 20200324135215.0 doi 10.3390/mi9030129 sideral 105453 ART-2018-105453 eng González, I. A label free disposable device for rapid isolation of rare tumor cells from blood by ultrasounds 2018 Access copy available to the general public Unrestricted The use of blood samples as liquid biopsy is a label-free method for cancer diagnosis that offers benefits over traditional invasive biopsy techniques. Cell sorting by acoustic waves offers a means to separate rare cells from blood samples based on their physical properties in a label-free, contactless and biocompatible manner. Herein, we describe a flow-through separation approach that provides an efficient separation of tumor cells (TCs) from white blood cells (WBCs) in a microfluidic device, "THINUS-Chip" (Thin-Ultrasonic-Separator-Chip), actuated by ultrasounds. We introduce for the first time the concept of plate acoustic waves (PAW) applied to acoustophoresis as a new strategy. It lies in the geometrical chip design: different to other microseparators based on either bulk acoustic waves (BAW) or surface waves (SAW, SSAW and tSAW), it allows the use of polymeric materials without restrictions in the frequency of work. We demonstrate its ability to perform high-throughput isolation of TCs from WBCs, allowing a recovery rate of 84%±8% of TCs with a purity higher than 80% and combined viability of 85% at a flow rate of 80 µL/min (4.8 mL/h). The THINUS-Chip performs cell fractionation with low-cost manufacturing processes, opening the door to possible easy printing fabrication. info:eu-repo/grantAgreement/ES/MINECO/BIO2011-30535-C04-01 info:eu-repo/grantAgreement/ES/MINECO/DPI2017-90147-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.426 2018 INSTRUMENTS & INSTRUMENTATION 25 / 61 = 0.41 2018 Q2 T2 NANOSCIENCE & NANOTECHNOLOGY 55 / 94 = 0.585 2018 Q3 T2 0.536 2018 Control and Systems Engineering 2018 Q2 Mechanical Engineering 2018 Q2 Electrical and Electronic Engineering 2018 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Earl, J. (orcid)0000-0001-5376-4440 Fernández, L.J. Universidad de Zaragoza Sainz, B. , Jr. Pinto, A. (orcid)0000-0003-0734-129X Monge, R. Alcalá, S. Castillejo, A. Soto, J.L. Carrato, A. 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 9, 3 (2018), 129 [15 pp] Micromachines (Basel) Micromachines 2072-666X 743517 http://zaguan.unizar.es/record/70053/files/texto_completo.pdf Versión publicada 107238 http://zaguan.unizar.es/record/70053/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:70053 articulos driver 2020-03-24-10:01:38 ARTICLE