70054 20250619084223.0 doi 10.1098/rsos.171592 sideral 105456 ART-2018-105456 eng Alves, A.P. Graph analysis of cell clusters forming vascular networks 2018 Access copy available to the general public Unrestricted This manuscript describes the experimental observation of vasculogenesis in chick embryos by means of network analysis. The formation of the vascular network was observed in the area opaca of embryos from 40 to 55 h of development. In the area opaca endothelial cell clusters self-organize as a primitive and approximately regular network of capillaries. The process was observed by bright-field microscopy in control embryos and in embryos treated with Bevacizumab (Avastin®), an antibody that inhibits the signalling of the vascular endothelial growth factor (VEGF). The sequence of images of the vascular growth were thresholded, and used to quantify the forming network in control and Avastin-treated embryos. This characterization is made by measuring vessels density, number of cell clusters and the largest cluster density. From the original images, the topology of the vascular network was extracted and characterized by means of the usual network metrics such as: the degree distribution, average clustering coefficient, average short path length and assortativity, among others. This analysis allows to monitor how the largest connected cluster of the vascular network evolves in time and provides with quantitative evidence of the disruptive effects that Avastin has on the tree structure of vascular networks. info:eu-repo/grantAgreement/ES/DGA/E19 info:eu-repo/grantAgreement/ES/MINECO/FIS2012-38266-C02-01 info:eu-repo/grantAgreement/ES/MINECO/FIS2014-55867-P info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.515 2018 MULTIDISCIPLINARY SCIENCES 24 / 68 = 0.353 2018 Q2 T2 1.131 2018 Multidisciplinary 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Mesquita, O.N. Gómez-Gardeñes, J. Universidad de Zaragoza (orcid)0000-0001-5204-1937 Agero, U. 2003 395 Universidad de Zaragoza Dpto. Física Materia Condensa. Área Física Materia Condensada 5, 3 (2018), 171592 [14 pp] R. Soc. open sci. Royal Society Open Science 2054-5703 1674382 http://zaguan.unizar.es/record/70054/files/texto_completo.pdf Versión publicada 70084 http://zaguan.unizar.es/record/70054/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:70054 articulos driver 2025-06-19-08:41:24 ARTICLE