000070207 001__ 70207 000070207 005__ 20240104111808.0 000070207 0247_ $$2doi$$a10.1017/S0022029917000425 000070207 0248_ $$2sideral$$a101691 000070207 037__ $$aART-2017-101691 000070207 041__ $$aeng 000070207 100__ $$aUffo, O. 000070207 245__ $$aAnalysis of microsatellite markers in a Cuban water buffalo breed 000070207 260__ $$c2017 000070207 5060_ $$aAccess copy available to the general public$$fUnrestricted 000070207 5203_ $$aThe aim of this Regional Research Communication was to validate a panel of 30 microsatellite markers recommended by FAO/ISAG for studies of biodiversity in cattle to improve the characterisation of Cuban buffalo populations. The water buffalo (Bubalus bubalis) is an economically important livestock species. Therefore, research focused on the study of the genetic relationships among water buffalo populations is useful to support conservation decisions and to design breeding schemes. Twenty-eight of the 30 tested regions were amplified, one of which (ETH10) turned out to be monomorphic. A total of 143 alleles were observed in the Cuban water buffalo population. The average number of alleles per locus was 5·04. The number of alleles per polymorphic locus ranged from two (INRA 63 and MM12) to nine (ETH185). The observed and expected heterozygosity ranged from 0·108 (HAUT24) to 0·851 (CSSM66) and 0·104 (MM12) to 0·829(INRA32), respectively. The polymorphic information content (PIC) ranged from 0·097 (MM12) to 0·806 (INRA32), and the overall value for these markers was 0·482. Within the population, inbreeding estimates (F IS) was positive in 14 of the 30 loci analysed. This study thus highlights the usefulness of heterologous bovine microsatellite markers to assess the genetic variability in Cuban water buffalo breeds. Furthermore, the results can be utilised for future breeding strategies and conservation. 000070207 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000070207 590__ $$a1.17$$b2017 000070207 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b23 / 59 = 0.39$$c2017$$dQ2$$eT2 000070207 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b87 / 133 = 0.654$$c2017$$dQ3$$eT2 000070207 592__ $$a0.573$$b2017 000070207 593__ $$aAnimal Science and Zoology$$c2017$$dQ2 000070207 593__ $$aMedicine (miscellaneous)$$c2017$$dQ2 000070207 593__ $$aFood Science$$c2017$$dQ2 000070207 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000070207 700__ $$aMartínez, N. 000070207 700__ $$aAcosta, A. 000070207 700__ $$0(orcid)0000-0001-6489-8399$$aSanz, A.$$uUniversidad de Zaragoza 000070207 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, I.$$uUniversidad de Zaragoza 000070207 700__ $$0(orcid)0000-0001-5687-6704$$aOsta, R.$$uUniversidad de Zaragoza 000070207 700__ $$0(orcid)0000-0003-3289-2675$$aRodellar, C.$$uUniversidad de Zaragoza 000070207 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, P.$$uUniversidad de Zaragoza 000070207 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética 000070207 773__ $$g84, 3 (2017), 289-292$$pJ. dairy res.$$tJOURNAL OF DAIRY RESEARCH$$x0022-0299 000070207 8564_ $$s346911$$uhttps://zaguan.unizar.es/record/70207/files/texto_completo.pdf$$yPostprint 000070207 8564_ $$s118073$$uhttps://zaguan.unizar.es/record/70207/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000070207 909CO $$ooai:zaguan.unizar.es:70207$$particulos$$pdriver 000070207 951__ $$a2024-01-04-11:01:14 000070207 980__ $$aARTICLE