70207 20240104111808.0 doi 10.1017/S0022029917000425 sideral 101691 ART-2017-101691 eng Uffo, O. Analysis of microsatellite markers in a Cuban water buffalo breed 2017 Access copy available to the general public Unrestricted The aim of this Regional Research Communication was to validate a panel of 30 microsatellite markers recommended by FAO/ISAG for studies of biodiversity in cattle to improve the characterisation of Cuban buffalo populations. The water buffalo (Bubalus bubalis) is an economically important livestock species. Therefore, research focused on the study of the genetic relationships among water buffalo populations is useful to support conservation decisions and to design breeding schemes. Twenty-eight of the 30 tested regions were amplified, one of which (ETH10) turned out to be monomorphic. A total of 143 alleles were observed in the Cuban water buffalo population. The average number of alleles per locus was 5·04. The number of alleles per polymorphic locus ranged from two (INRA 63 and MM12) to nine (ETH185). The observed and expected heterozygosity ranged from 0·108 (HAUT24) to 0·851 (CSSM66) and 0·104 (MM12) to 0·829(INRA32), respectively. The polymorphic information content (PIC) ranged from 0·097 (MM12) to 0·806 (INRA32), and the overall value for these markers was 0·482. Within the population, inbreeding estimates (F IS) was positive in 14 of the 30 loci analysed. This study thus highlights the usefulness of heterologous bovine microsatellite markers to assess the genetic variability in Cuban water buffalo breeds. Furthermore, the results can be utilised for future breeding strategies and conservation. info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 1.17 2017 AGRICULTURE, DAIRY & ANIMAL SCIENCE 23 / 59 = 0.39 2017 Q2 T2 FOOD SCIENCE & TECHNOLOGY 87 / 133 = 0.654 2017 Q3 T2 0.573 2017 Animal Science and Zoology 2017 Q2 Medicine (miscellaneous) 2017 Q2 Food Science 2017 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Martínez, N. Acosta, A. Sanz, A. Universidad de Zaragoza (orcid)0000-0001-6489-8399 Martín-Burriel, I. Universidad de Zaragoza (orcid)0000-0001-6016-4726 Osta, R. Universidad de Zaragoza (orcid)0000-0001-5687-6704 Rodellar, C. Universidad de Zaragoza (orcid)0000-0003-3289-2675 Zaragoza, P. Universidad de Zaragoza (orcid)0000-0001-5740-0185 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 84, 3 (2017), 289-292 J. dairy res. JOURNAL OF DAIRY RESEARCH 0022-0299 346911 http://zaguan.unizar.es/record/70207/files/texto_completo.pdf Postprint 118073 http://zaguan.unizar.es/record/70207/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:70207 articulos driver 2024-01-04-11:01:14 ARTICLE