000070280 001__ 70280
000070280 005__ 20200103141023.0
000070280 0247_ $$2doi$$a10.1186/s13567-018-0528-8
000070280 0248_ $$2sideral$$a105902
000070280 037__ $$aART-2018-105902
000070280 041__ $$aeng
000070280 100__ $$0(orcid)0000-0003-3016-9125$$aPitarch, J.L.
000070280 245__ $$aLow sequence diversity of the prion protein gene (PRNP) in wild deer and goat species from Spain
000070280 260__ $$c2018
000070280 5060_ $$aAccess copy available to the general public$$fUnrestricted
000070280 5203_ $$aThe first European cases of chronic wasting disease (CWD) in free-ranging reindeer and wild elk were confirmed in Norway in 2016 highlighting the urgent need to understand transmissible spongiform encephalopathies (TSEs) in the context of European deer species and the many individual populations throughout the European continent. The genetics of the prion protein gene (PRNP) are crucial in determining the relative susceptibility to TSEs. To establish PRNP gene sequence diversity for free-ranging ruminants in the Northeast of Spain, the open reading frame was sequenced in over 350 samples from five species: Iberian red deer (Cervus elaphus hispanicus), roe deer (Capreolus capreolus), fallow deer (Dama dama), Iberian wild goat (Capra pyrenaica hispanica) and Pyrenean chamois (Rupicapra p. pyrenaica). Three single nucleotide polymorphisms (SNPs) were found in red deer: a silent mutation at codon 136, and amino acid changes T98A and Q226E. Pyrenean chamois revealed a silent SNP at codon 38 and an allele with a single octapeptide-repeat deletion. No polymorphisms were found in roe deer, fallow deer and Iberian wild goat. This apparently low variability of the PRNP coding region sequences of four major species in Spain resembles previous findings for wild mammals, but implies that larger surveys will be necessary to find novel, low frequency PRNP gene alleles that may be utilized in CWD risk control.
000070280 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000070280 590__ $$a3.117$$b2018
000070280 591__ $$aVETERINARY SCIENCES$$b4 / 141 = 0.028$$c2018$$dQ1$$eT1
000070280 592__ $$a0.124$$b2018
000070280 593__ $$aVeterinary (miscellaneous)$$c2018$$dQ4
000070280 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000070280 700__ $$aRaksa, H.C.
000070280 700__ $$0(orcid)0000-0002-6770-3400$$aArnal, M.C.$$uUniversidad de Zaragoza
000070280 700__ $$0(orcid)0000-0002-1592-5052$$aRevilla, M.$$uUniversidad de Zaragoza
000070280 700__ $$0(orcid)0000-0003-2208-1350$$aMartínez, D.$$uUniversidad de Zaragoza
000070280 700__ $$0(orcid)0000-0003-3289-4267$$aFernández De Luco, D.$$uUniversidad de Zaragoza
000070280 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, J.J.$$uUniversidad de Zaragoza
000070280 700__ $$aGoldmann, W.
000070280 700__ $$0(orcid)0000-0001-5105-6133$$aAcín, C.$$uUniversidad de Zaragoza
000070280 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000070280 7102_ $$11009$$2X$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Técnica. Lab. y Talleres
000070280 773__ $$g49, 1 (2018), 33 [7 pp]$$pVet. res.$$tVeterinary Research$$x0928-4249
000070280 8564_ $$s1333939$$uhttps://zaguan.unizar.es/record/70280/files/texto_completo.pdf$$yVersión publicada
000070280 8564_ $$s107913$$uhttps://zaguan.unizar.es/record/70280/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000070280 909CO $$ooai:zaguan.unizar.es:70280$$particulos$$pdriver
000070280 951__ $$a2020-01-03-14:03:04
000070280 980__ $$aARTICLE