70280 20200103141023.0 doi 10.1186/s13567-018-0528-8 sideral 105902 ART-2018-105902 eng (orcid)0000-0003-3016-9125 Pitarch, J.L. Low sequence diversity of the prion protein gene (PRNP) in wild deer and goat species from Spain 2018 Access copy available to the general public Unrestricted The first European cases of chronic wasting disease (CWD) in free-ranging reindeer and wild elk were confirmed in Norway in 2016 highlighting the urgent need to understand transmissible spongiform encephalopathies (TSEs) in the context of European deer species and the many individual populations throughout the European continent. The genetics of the prion protein gene (PRNP) are crucial in determining the relative susceptibility to TSEs. To establish PRNP gene sequence diversity for free-ranging ruminants in the Northeast of Spain, the open reading frame was sequenced in over 350 samples from five species: Iberian red deer (Cervus elaphus hispanicus), roe deer (Capreolus capreolus), fallow deer (Dama dama), Iberian wild goat (Capra pyrenaica hispanica) and Pyrenean chamois (Rupicapra p. pyrenaica). Three single nucleotide polymorphisms (SNPs) were found in red deer: a silent mutation at codon 136, and amino acid changes T98A and Q226E. Pyrenean chamois revealed a silent SNP at codon 38 and an allele with a single octapeptide-repeat deletion. No polymorphisms were found in roe deer, fallow deer and Iberian wild goat. This apparently low variability of the PRNP coding region sequences of four major species in Spain resembles previous findings for wild mammals, but implies that larger surveys will be necessary to find novel, low frequency PRNP gene alleles that may be utilized in CWD risk control. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.117 2018 VETERINARY SCIENCES 4 / 141 = 0.028 2018 Q1 T1 0.124 2018 Veterinary (miscellaneous) 2018 Q4 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Raksa, H.C. (orcid)0000-0002-6770-3400 Arnal, M.C. Universidad de Zaragoza (orcid)0000-0002-1592-5052 Revilla, M. Universidad de Zaragoza (orcid)0000-0003-2208-1350 Martínez, D. Universidad de Zaragoza (orcid)0000-0003-3289-4267 Fernández De Luco, D. Universidad de Zaragoza (orcid)0000-0002-7173-7216 Badiola, J.J. Universidad de Zaragoza Goldmann, W. (orcid)0000-0001-5105-6133 Acín, C. Universidad de Zaragoza 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1009 X Universidad de Zaragoza Dpto. Patología Animal Área Técnica. Lab. y Talleres 49, 1 (2018), 33 [7 pp] Vet. res. Veterinary Research 0928-4249 1333939 http://zaguan.unizar.es/record/70280/files/texto_completo.pdf Versión publicada 107913 http://zaguan.unizar.es/record/70280/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:70280 articulos driver 2020-01-03-14:03:04 ARTICLE