70281 20200103141024.0 doi 10.3390/ma11040580 sideral 105903 ART-2018-105903 eng Batool, F. Synthesis of a novel electrospun polycaprolactone scaffold functionalized with ibuprofen for periodontal regeneration: An in vitro and in vivo study 2018 Access copy available to the general public Unrestricted Ibuprofen (IBU) has been shown to improve periodontal treatment outcomes. The aimof this study was to develop a new anti-inflammatory scaffold by functionalizing an electrospun nanofibrous poly-e-caprolactone membrane with IBU (IBU-PCL) and to evaluate its impact on periodontal inflammation, wound healing and regeneration in vitro and in vivo. IBU-PCL was synthesized through electrospinning. The effects of IBU-PCL on the proliferation and migration of epithelial cells (EC) and fibroblasts (FB) exposed to Porphyromonas gingivlais lipopolysaccharide (Pg-LPS) were evaluated through the AlamarBlue test and scratch assay, respectively. Anti-inflammatory and remodeling properties were investigated through Real time qPCR. Finally, the in vivo efficacy of the IBU-PCL membrane was assessed in an experimental periodontitis mouse model through histomorphometric analysis. The results showed that the anti-inflammatory effects of IBU on gingival cells were effectively amplified using the functionalizedmembrane. IBU-PCL reduced the proliferation and migration of cells challenged by Pg-LPS, as well as the expression of fibronectin-1, collagen-IV, integrin a3ß1 and laminin-5. In vivo, the membranes significantly improved the clinical attachment and IBU-PCL also reduced inflammation-induced bone destruction. These data showed that the IBU-PCL membrane could efficiently and differentially control inflammatory and migratory gingival cell responses and potentially promote periodontal regeneration. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.972 2018 MATERIALS SCIENCE, MULTIDISCIPLINARY 102 / 293 = 0.348 2018 Q2 T2 0.686 2018 Materials Science (miscellaneous) 2018 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Morand, D.-N. Thomas, L. Bugueno, I.M. Aragon, J. Universidad de Zaragoza (orcid)0000-0002-2966-9088 Irusta, S. Universidad de Zaragoza Keller, L. Benkirane-Jessel, N. Tenenbaum, H. Huck, O. 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 11, 4 (2018), 580 [18 pp] MATERIALS Materials 1996-1944 600887 http://zaguan.unizar.es/record/70281/files/texto_completo.pdf Versión publicada 97866 http://zaguan.unizar.es/record/70281/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:70281 articulos driver 2020-01-03-14:03:06 ARTICLE