Self-sustained enzymatic cascade for the production of 2, 5-furandicarboxylic acid from 5-methoxymethylfurfural

Carro, J. ; Fernández-Fueyo, E. ; Fernández-Alonso, C. ; Cañada, J. ; Ullrich, R. ; Hofrichter, M. ; Alcalde, M. ; Ferreira, P. (Universidad de Zaragoza) ; Martínez, A.T.

Resumen: Background: 2, 5-Furandicarboxylic acid is a renewable building block for the production of polyfurandicarboxylates, which are biodegradable polyesters expected to substitute their classical counterparts derived from fossil resources. It may be produced from bio-based 5-hydroxymethylfurfural or 5-methoxymethylfurfural, both obtained by the acidic dehydration of biomass-derived fructose. 5-Methoxymethylfurfural, which is produced in the presence of methanol, generates less by-products and exhibits better storage stability than 5-hydroxymethylfurfural being, therefore, the industrial substrate of choice.
Results: In this work, an enzymatic cascade involving three fungal oxidoreductases has been developed for the production of 2, 5-furandicarboxylic acid from 5-methoxymethylfurfural. Aryl-alcohol oxidase and unspecific peroxygenase act on 5-methoxymethylfurfural and its partially oxidized derivatives yielding 2, 5-furandicarboxylic acid, as well as methanol as a by-product. Methanol oxidase takes advantage of the methanol released for in situ producing H2O2 that, along with that produced by aryl-alcohol oxidase, fuels the peroxygenase reactions. In this way, the enzymatic cascade proceeds independently, with the only input of atmospheric O2, to attain a 70% conversion of initial 5-methoxymethylfurfural. The addition of some exogenous methanol to the reaction further improves the yield to attain an almost complete conversion of 5-methoxymethylfurfural into 2, 5-furandicarboxylic acid.
Conclusions: The synergistic action of aryl-alcohol oxidase and unspecific peroxygenase in the presence of 5-methoxymethylfurfural and O2 is sufficient for the production of 2, 5-furandicarboxylic acid. The addition of methanol oxidase to the enzymatic cascade increases the 2, 5-furandicarboxylic acid yields by oxidizing a reaction by-product to fuel the peroxygenase reactions.

Idioma: Inglés

DOI: 10.1186/s13068-018-1091-2

Año: 2018

Publicado en: Biotechnology for Biofuels 11, 1 (2018), 86 [10 pp]
ISSN: 1754-6834

Factor impacto JCR: 5.452 (2018)

Categ. JCR: ENERGY & FUELS rank: 16 / 103 = 0.155 (2018) - Q1 - T1
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 18 / 162 = 0.111 (2018) - Q1 - T1

Factor impacto SCIMAGO: 1.762 - Applied Microbiology and Biotechnology (Q1) - Biotechnology (Q1) - Renewable Energy, Sustainability and the Environment (Q1) - Management, Monitoring, Policy and Law (Q1) - Energy (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/MEC/FPU-AP2012-2041

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)