Long time remodeling during retinal degeneration evaluated by optical coherence tomography, immunocytochemistry and fundus autofluorescence

Pinilla, Isabel (Universidad de Zaragoza) ; Fernández Sánchez, Laura ; Segura, Francisco Javier (Universidad de Zaragoza) ; Sánchez Cano, Ana Isabel (Universidad de Zaragoza) ; Tamarit, José María ; Fuentes Broto, Lorena (Universidad de Zaragoza) ; Eells, Jt ; Lax, Pedro ; Cuenca, Nicolás

Resumen: Purpose: To characterize the relationship between fundus autofluorescence (FAF), Optical Coherence Tomography (OCT) and immuno histo chemistry (IHC) over the course of chronic retinal degeneration in the P23H rat. Methods: Homozygous albino P23H rats, SpragueeDawley (SD) rats as controls and pigmented Long Evans (LE) rats were used. A Spectralis HRA OCT system was used for scanning laser ophthalmoscopy
(SLO) imaging OCT and angiography. To determine FAF, fluorescence was excited using diode laser at 488 nm. A fast retina map OCT was performed using the optic nerve as a landmark. IHC was performed to correlate with the findings of OCT and FAF changes.
Results: During the course of retinal degeneration, the FAF pattern evolved from some spotting at 2 months old to a mosaic of hyper fluorescent dots in rats 6 months and older. Retinal thicknesses progressively diminished over the course of the disease. At later stages of degeneration, OCT documented changes in the retinal layers, however, IHC better identified the cell loss and remodeling changes.
Angiography revealed attenuation of the retinal vascular plexus with time.
Conclusion: We provide for the first time a detailed long-term analysis of the course of retinal degeneration in P23H rats using a combination of SLO and OCT imaging, angiography, FAF and IHC. Although, the application of noninvasive methods enables longitudinal studies and will decrease thenumber of animals needed for a study, IHC is still an essential tool to identify retinal changes at the cellular level.

Idioma: Inglés

DOI: 10.1016/j.exer.2015.10.012

Año: 2016

Publicado en: Experimental Eye Research 150 (2016), 122-134
ISSN: 0014-4835

Factor impacto JCR: 3.332 (2016)

Categ. JCR: OPHTHALMOLOGY rank: 10 / 59 = 0.169 (2016) - Q1 - T1

Factor impacto SCIMAGO: 1.331 - Ophthalmology (Q1) - Sensory Systems (Q2) - Cellular and Molecular Neuroscience (Q3)

Financiación: info:eu-repo/grantAgreement/ES/DGA/B99
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/PI13-01124
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/RETICS-RD12-0034-0010
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BFU2012-36845
Financiación: info:eu-repo/grantAgreement/ES/UZ/UZ2011-BIO-02

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Área Fisiología (Dpto. Farmacología y Fisiolog.)
Área (Departamento): Área Oftalmología (Dpto. Cirugía,Ginecol.Obstetr.)
Área (Departamento): Área Óptica (Dpto. Física Aplicada)