70591 20200221144300.0 doi 10.1016/j.exer.2015.10.012 sideral 93944 ART-2016-93944 eng (orcid)0000-0003-0349-9997 Pinilla, Isabel Universidad de Zaragoza Long time remodeling during retinal degeneration evaluated by optical coherence tomography, immunocytochemistry and fundus autofluorescence 2016 Access copy available to the general public Unrestricted Purpose: To characterize the relationship between fundus autofluorescence (FAF), Optical Coherence Tomography (OCT) and immuno histo chemistry (IHC) over the course of chronic retinal degeneration in the P23H rat. Methods: Homozygous albino P23H rats, SpragueeDawley (SD) rats as controls and pigmented Long Evans (LE) rats were used. A Spectralis HRA OCT system was used for scanning laser ophthalmoscopy (SLO) imaging OCT and angiography. To determine FAF, fluorescence was excited using diode laser at 488 nm. A fast retina map OCT was performed using the optic nerve as a landmark. IHC was performed to correlate with the findings of OCT and FAF changes. Results: During the course of retinal degeneration, the FAF pattern evolved from some spotting at 2 months old to a mosaic of hyper fluorescent dots in rats 6 months and older. Retinal thicknesses progressively diminished over the course of the disease. At later stages of degeneration, OCT documented changes in the retinal layers, however, IHC better identified the cell loss and remodeling changes. Angiography revealed attenuation of the retinal vascular plexus with time. Conclusion: We provide for the first time a detailed long-term analysis of the course of retinal degeneration in P23H rats using a combination of SLO and OCT imaging, angiography, FAF and IHC. Although, the application of noninvasive methods enables longitudinal studies and will decrease thenumber of animals needed for a study, IHC is still an essential tool to identify retinal changes at the cellular level. info:eu-repo/grantAgreement/ES/DGA/B99 info:eu-repo/grantAgreement/ES/ISCIII/PI13-01124 info:eu-repo/grantAgreement/ES/ISCIII/RETICS-RD12-0034-0010 info:eu-repo/grantAgreement/ES/MINECO/BFU2012-36845 info:eu-repo/grantAgreement/ES/UZ/UZ2011-BIO-02 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 3.332 2016 OPHTHALMOLOGY 10 / 59 = 0.169 2016 Q1 T1 1.331 2016 Ophthalmology 2016 Q1 Sensory Systems 2016 Q2 Cellular and Molecular Neuroscience 2016 Q3 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Fernández Sánchez, Laura (orcid)0000-0002-9250-9060 Segura, Francisco Javier Universidad de Zaragoza (orcid)0000-0002-5621-1937 Sánchez Cano, Ana Isabel Universidad de Zaragoza Tamarit, José María (orcid)0000-0003-2656-6750 Fuentes Broto, Lorena Universidad de Zaragoza Eells, Jt Lax, Pedro Cuenca, Nicolás 1005 410 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Fisiología 1004 646 Universidad de Zaragoza Dpto. Cirugía,Ginecol.Obstetr. Área Oftalmología 2002 647 Universidad de Zaragoza Dpto. Física Aplicada Área Óptica 150 (2016), 122-134 Exp. Eye Res. Experimental Eye Research 0014-4835 547538 http://zaguan.unizar.es/record/70591/files/texto_completo.pdf Postprint 58525 http://zaguan.unizar.es/record/70591/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:70591 articulos driver 2020-02-21-13:32:14 ARTICLE