000070597 001__ 70597
000070597 005__ 20200221144242.0
000070597 0247_ $$2doi$$a10.1002/stem.2414
000070597 0248_ $$2sideral$$a106061
000070597 037__ $$aART-2016-106061
000070597 041__ $$aeng
000070597 100__ $$aCapowski, E.E.
000070597 245__ $$aRegulation of WNT Signaling by VSX2 During Optic Vesicle Patterning in Human Induced Pluripotent Stem Cells
000070597 260__ $$c2016
000070597 5060_ $$aAccess copy available to the general public$$fUnrestricted
000070597 5203_ $$aFew gene targets of Visual System Homeobox 2 (VSX2) have been identified despite its broad and critical role in the maintenance of neural retina (NR) fate during early retinogenesis. We performed VSX2 ChIP-seq and ChIP-PCR assays on early stage optic vesicle-like structures (OVs) derived from human iPS cells (hiPSCs), which highlighted WNT pathway genes as direct regulatory targets of VSX2. Examination of early NR patterning in hiPSC-OVs from a patient with a functional null mutation in VSX2 revealed mis-expression and upregulation of WNT pathway components and retinal pigmented epithelium (RPE) markers in comparison to control hiPSCOVs. Furthermore, pharmacological inhibition of WNT signaling rescued the early mutant phenotype, whereas augmentation of WNT signaling in control hiPSC-OVs phenocopied the mutant. These findings reveal an important role for VSX2 as a regulator of WNT signaling and suggest that VSX2 may act to maintain NR identity at the expense of RPE in part by direct repression of WNT pathway constituents.
000070597 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000070597 590__ $$a5.599$$b2016
000070597 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b16 / 160 = 0.1$$c2016$$dQ1$$eT1
000070597 591__ $$aCELL BIOLOGY$$b43 / 189 = 0.228$$c2016$$dQ1$$eT1
000070597 591__ $$aCELL & TISSUE ENGINEERING$$b4 / 21 = 0.19$$c2016$$dQ1$$eT1
000070597 591__ $$aONCOLOGY$$b37 / 217 = 0.171$$c2016$$dQ1$$eT1
000070597 591__ $$aHEMATOLOGY$$b12 / 70 = 0.171$$c2016$$dQ1$$eT1
000070597 592__ $$a2.971$$b2016
000070597 593__ $$aCell Biology$$c2016$$dQ1
000070597 593__ $$aMolecular Medicine$$c2016$$dQ1
000070597 593__ $$aMedicine (miscellaneous)$$c2016$$dQ1
000070597 593__ $$aDevelopmental Biology$$c2016$$dQ1
000070597 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000070597 700__ $$aWright, L.S.
000070597 700__ $$aLiang, K.
000070597 700__ $$aPhillips, M.J.
000070597 700__ $$aWallace, K.
000070597 700__ $$aPetelinsek, A.
000070597 700__ $$aHagstrom, A.
000070597 700__ $$0(orcid)0000-0003-0349-9997$$aPinilla, I.$$uUniversidad de Zaragoza
000070597 700__ $$aBorys, K.
000070597 700__ $$aLien, J.
000070597 700__ $$aMin, J.H.
000070597 700__ $$aKeles, S.
000070597 700__ $$aThomson, J.A.
000070597 700__ $$aGamm, D.M.
000070597 7102_ $$11004$$2646$$aUniversidad de Zaragoza$$bDpto. Cirugía,Ginecol.Obstetr.$$cÁrea Oftalmología
000070597 773__ $$g34, 11 (2016), 2625-2634$$pStem cells$$tSTEM CELLS$$x1066-5099
000070597 8564_ $$s867623$$uhttps://zaguan.unizar.es/record/70597/files/texto_completo.pdf$$yVersión publicada
000070597 8564_ $$s117466$$uhttps://zaguan.unizar.es/record/70597/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000070597 909CO $$ooai:zaguan.unizar.es:70597$$particulos$$pdriver
000070597 951__ $$a2020-02-21-13:22:15
000070597 980__ $$aARTICLE