000070600 001__ 70600 000070600 005__ 20200910145137.0 000070600 0247_ $$2doi$$a10.7717/peerj.2407 000070600 0248_ $$2sideral$$a106064 000070600 037__ $$aART-2016-106064 000070600 041__ $$aeng 000070600 100__ $$aShiposha, V. 000070600 245__ $$aGenetic structure and diversity of the selfing model grass Brachypodium stacei (Poaceae) in Western Mediterranean: out of the Iberian Peninsula and into the islands 000070600 260__ $$c2016 000070600 5060_ $$aAccess copy available to the general public$$fUnrestricted 000070600 5203_ $$aAnnual Mediterranean species of the genus Brachypodiurn are promising model plants for energy crops since their selfing nature and short-life cycles are an advantage in breeding programs. The false brome, B. distachyon, has already been sequenced and new genomic initiatives have triggered the de-novo genuine sequencing of its close relatives such as B. stacei, a species that was until recently mistaken for B. distachyon. However, the success of these initiatives hinges on detailed knowledge about the distribution of genetic variation within and among populations for the effective use of germplasm in la breeding program. Understanding population genetic diversity and genetic structure is also an important prerequisite for designing effective experimental populations for genomic wide studies. However, population genetic data are still limited in B. stacei. We therefore selected and amplified 10 nuclear microsatellite markers to depict patterns of population structure and genetic variation 13 among 181 individuals from 19 populations of B. stacei occurring in its predominant range, the western Mediterranean area: mainland Iberian Peninsula, continental Balearic Islands and oceanic Canary Islands. Our genetic results support the occurrence of a predominant selfing system with extremely high levels of homozygosity across the analyzed populations. Despite the low level of genetic variation found, two different genetic clusters were retrieved, one clustering all SE Iberian mainland populations and the island of Minorca and another one grouping all SE Iberian mainland populations, the Canary Islands and all Majorcan populations except one that clustered with the former group. These results, together with a high sharing of alleles (89%) suggest different colonization routes from the mainland Iberian Peninsula into the islands. A recent colonization scenario could explain the relatively low levels of genetic diversity and low number of alleles found in the Canary Islands populations while older colonization events are hypothesized to explain the high genetic diversity values found in the Majorcan populations. Our study provides widely applicable information about geographical patterns of genetic variation in B. stacei. Among others, the genetic pattern and the existence of local alleles will need to be adequately reflected in the germplasm collection of B. stacei for efficient genome wide association studies. 000070600 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/CGL2012-39953-C02-01$$9info:eu-repo/grantAgreement/EC/FP7/301257/EU/The model plant system Trithuria (Hydatellaceae), a new window into the origin of flowering plants and gene function/ORIGIN$$9info:eu-repo/grantAgreement/ES/DGA/Bioflora 000070600 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000070600 590__ $$a2.177$$b2016 000070600 591__ $$aMULTIDISCIPLINARY SCIENCES$$b19 / 63 = 0.302$$c2016$$dQ2$$eT1 000070600 592__ $$a1.124$$b2016 000070600 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2016$$dQ1 000070600 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2016$$dQ1 000070600 593__ $$aMedicine (miscellaneous)$$c2016$$dQ1 000070600 593__ $$aNeuroscience (miscellaneous)$$c2016$$dQ2 000070600 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000070600 700__ $$0(orcid)0000-0001-7793-5259$$aCatalan, P.$$uUniversidad de Zaragoza 000070600 700__ $$aOlonova, M. 000070600 700__ $$aMarques, I. 000070600 7102_ $$15011$$2063$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Botánica 000070600 773__ $$g4 (2016), [23 pp.]$$pPeerJ$$tPeerJ$$x2167-8359 000070600 8564_ $$s1711443$$uhttps://zaguan.unizar.es/record/70600/files/texto_completo.pdf$$yVersión publicada 000070600 8564_ $$s104449$$uhttps://zaguan.unizar.es/record/70600/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000070600 909CO $$ooai:zaguan.unizar.es:70600$$particulos$$pdriver 000070600 951__ $$a2020-09-10-14:38:42 000070600 980__ $$aARTICLE