70639 20211216131131.0 doi 10.1088/2050-6120/4/4/042005 sideral 106012 ART-2016-106012 eng Galban, J. Universidad de Zaragoza (orcid)0000-0002-8973-5104 The intrinsic fluorescence of FAD and its application in analytical chemistry: a review 2016 Access copy available to the general public Unrestricted This review (with 106 references) mainly deals with the analytical applications of flavin-adenine dinucleotide (FAD) fluorescence. In the first section, the spectroscopic properties of this compound are reviewed at the light of his different acid-base, oxidation and structural forms; the chemical and spectroscopic properties of flavin mononucleotide (FMN) and other flavins will be also briefly discussed. The second section discusses how the properties of FAD fluorescence changes in flavoenzymes (FvEs), again considering the different chemical and structural forms; the glucose oxidase (GOx) and the choline oxidase (ChOx) cases will be commented. Since almost certainly the most reported analytical application of FAD fluorescence is as an auto-indicator in enzymatic methods catalysed by FvE oxidoreductases, it is important to know how the concentrations of the different forms of FAD changes along the reaction and, consequently, the fluorescence and the analytical signals. An approach to do this will be presented in section 3. The fourth part of the paper compiles the analytical applications which have been reported until now based in these fluorescence properties. Finally, some suggestions about tentative future research are also given. info:eu-repo/grantAgreement/ES/DGA/E74 info:eu-repo/grantAgreement/ES/MINECO/CTQ2012-34774 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 2.656 2016 CHEMISTRY, PHYSICAL 66 / 145 = 0.455 2016 Q2 T2 CHEMISTRY, ANALYTICAL 27 / 76 = 0.355 2016 Q2 T2 0.895 2016 Atomic and Molecular Physics, and Optics 2016 Q1 Instrumentation 2016 Q1 Materials Science (miscellaneous) 2016 Q1 Spectroscopy 2016 Q2 info:eu-repo/semantics/review info:eu-repo/semantics/publishedVersion Sanz-Vicente, I. Universidad de Zaragoza (orcid)0000-0002-3906-4576 Navarro, J. Universidad de Zaragoza de Marcos, S. Universidad de Zaragoza (orcid)0000-0002-7902-6005 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 4, 4 (2016), 042005 [19 pp] Methods appl. fluoresc. Methods and Applications in Fluorescence 2050-6120 1364004 http://zaguan.unizar.es/record/70639/files/texto_completo.pdf Versión publicada 106184 http://zaguan.unizar.es/record/70639/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:70639 articulos driver 2021-12-16-13:01:51 ARTICLE