70687 20190709135422.0 doi 10.1016/j.jinorgbio.2016.11.009 sideral 97109 ART-2017-97109 eng Sánchez-de-Diego, C. The anticancer effect related to disturbances in redox balance on Caco-2 cells caused by an alkynyl gold(I) complex 2017 Access copy available to the general public Unrestricted The alkynyl gold(I) derivative Au(C=CPh)(PTA)] (PTA = 1, 3, 5-triaza-7-phosphaadamantane) induces apoptosis in colorectal carcinoma tumour cells (Caco-2) without affecting to normal enterocytes. Au(C=CPh)(PTA)] is a slight lipophilic drug, stable in PBS (Phosphate Buffered Saline) and able to bind BSA (Bovin Serum Albumin) by hydrophobic interactions. Once inside the cell, Au(C=CPh)(PTA)] targets seleno proteins such as Thioredoxin Reductase 1, increasing ROS (Reactive Oxygen Species) levels, reducing cell viability and proliferation and inducing mitochondrial apoptotic pathway, pro-apoptotic and anti-apoptotic protein imbalance, loss of mitochondrial membrane potential, cytochrome c release and activation of caspases 9 and 3. Moreover, unlike other metal-based drugs such as cisplatin, Au(C=CPh)(PTA)] does not target nucleic acid, reducing the risk of side mutation in the DNA. In consequence, our results predict a promising future for Au(C=CPh)(PTA)] as a chemotherapeutic agent for colorectal carcinoma. info:eu-repo/grantAgreement/ES/DGA/A32 info:eu-repo/grantAgreement/ES/DGA/E104 info:eu-repo/grantAgreement/ES/ISCIII/CB06-03-1012 info:eu-repo/grantAgreement/ES/MINECO-FEDER/CTQ2013-48635-C2-1-P info:eu-repo/grantAgreement/ES/MINECO/SAF2013-41651-R info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 3.063 2017 CHEMISTRY, INORGANIC & NUCLEAR 10 / 45 = 0.222 2017 Q1 T1 BIOCHEMISTRY & MOLECULAR BIOLOGY 137 / 292 = 0.469 2017 Q2 T2 0.743 2017 Inorganic Chemistry 2017 Q2 Biochemistry 2017 Q3 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Mármol, I. Pérez, R. Gascón, S. Universidad de Zaragoza (orcid)0000-0002-3595-7668 Rodriguez-Yoldi, M. Universidad de Zaragoza (orcid)0000-0003-2457-3674 Cerrada, E. Universidad de Zaragoza 2010 760 Universidad de Zaragoza Dpto. Química Inorgánica Área Química Inorgánica 1005 410 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Fisiología 1009 X Universidad de Zaragoza Dpto. Patología Animal Área Técnica. Lab. y Talleres 166 (2017), 108-121 J. inorg. biochem. JOURNAL OF INORGANIC BIOCHEMISTRY 0162-0134 788742 http://zaguan.unizar.es/record/70687/files/texto_completo.pdf Postprint 45669 http://zaguan.unizar.es/record/70687/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:70687 articulos driver 2019-07-09-11:27:20 ARTICLE