000070694 001__ 70694
000070694 005__ 20220621094623.0
000070694 0247_ $$2doi$$a10.3389/fped.2017.00112
000070694 0248_ $$2sideral$$a104808
000070694 037__ $$aART-2017-104808
000070694 041__ $$aeng
000070694 100__ $$aLei, J.
000070694 245__ $$aUmbilical cord blood NOS1 as a potential biomarker of neonatal encephalopathy
000070694 260__ $$c2017
000070694 5060_ $$aAccess copy available to the general public$$fUnrestricted
000070694 5203_ $$aBackground: There are no definitive markers to aid in diagnosis of neonatal encephalopathy (NE). The purpose of our study was (1) to identify and evaluate the utility of neuronal nitric oxide synthase (NOS1) in umbilical cord blood as a NE biomarker and (2) to identify the source of NOS1 in umbilical cord blood. Methods: This was a nested case-control study of neonates > 35 weeks of gestation. ELISA for NOS1 in umbilical cord blood was performed. Sources of NOS1 in umbilical cord were investigated by immunohistochemistry, western blot, ELISA, and quantitative PCR. Furthermore, umbilical cords of full-term neonates were subjected to 1% hypoxia ex vivo. Results: NOS1 was present in umbilical cord blood and increased in NE cases compared with controls. NOS1 was expressed in endothelial cells of the umbilical cord vein, but not in artery or blood cells. In ex vivo experiments, hypoxia was associated with increased levels of NOS1 in venous endothelial cells of the umbilical cord as well as in ex vivo culture medium. Conclusion: This is the first study to investigate an early marker of NE. NOS1 is elevated with hypoxia, and further studies are needed to investigate it as a valuable tool for early diagnosis of neonatal brain injury.
000070694 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000070694 590__ $$a2.335$$b2017
000070694 591__ $$aPEDIATRICS$$b38 / 124 = 0.306$$c2017$$dQ2$$eT1
000070694 592__ $$a0.0$$b2017
000070694 593__ $$aPediatrics, Perinatology and Child Health$$c2017
000070694 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000070694 700__ $$aPaules, C.
000070694 700__ $$aNigrini, E.
000070694 700__ $$aRosenzweig, J.M.
000070694 700__ $$aBahabry, R.
000070694 700__ $$aFarzin, A.
000070694 700__ $$aYang, S.
000070694 700__ $$aNorthington, F.J.
000070694 700__ $$0(orcid)0000-0002-8476-9161$$aOros, D.$$uUniversidad de Zaragoza
000070694 700__ $$aMcKenney, S.
000070694 700__ $$aJohnston, M.V.
000070694 700__ $$aGraham, E.M.
000070694 700__ $$aBurd, I.
000070694 7102_ $$11004$$2645$$aUniversidad de Zaragoza$$bDpto. Cirugía,Ginecol.Obstetr.$$cÁrea Obstetricia y Ginecología
000070694 773__ $$g5 (2017), 112$$pFront. pediatr.$$tFRONTIERS IN PEDIATRICS$$x2296-2360
000070694 8564_ $$s301374$$uhttps://zaguan.unizar.es/record/70694/files/texto_completo.pdf$$yVersión publicada
000070694 8564_ $$s90819$$uhttps://zaguan.unizar.es/record/70694/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000070694 909CO $$ooai:zaguan.unizar.es:70694$$particulos$$pdriver
000070694 951__ $$a2022-06-21-09:40:01
000070694 980__ $$aARTICLE