70694 20220621094623.0 doi 10.3389/fped.2017.00112 sideral 104808 ART-2017-104808 eng Lei, J. Umbilical cord blood NOS1 as a potential biomarker of neonatal encephalopathy 2017 Access copy available to the general public Unrestricted Background: There are no definitive markers to aid in diagnosis of neonatal encephalopathy (NE). The purpose of our study was (1) to identify and evaluate the utility of neuronal nitric oxide synthase (NOS1) in umbilical cord blood as a NE biomarker and (2) to identify the source of NOS1 in umbilical cord blood. Methods: This was a nested case-control study of neonates > 35 weeks of gestation. ELISA for NOS1 in umbilical cord blood was performed. Sources of NOS1 in umbilical cord were investigated by immunohistochemistry, western blot, ELISA, and quantitative PCR. Furthermore, umbilical cords of full-term neonates were subjected to 1% hypoxia ex vivo. Results: NOS1 was present in umbilical cord blood and increased in NE cases compared with controls. NOS1 was expressed in endothelial cells of the umbilical cord vein, but not in artery or blood cells. In ex vivo experiments, hypoxia was associated with increased levels of NOS1 in venous endothelial cells of the umbilical cord as well as in ex vivo culture medium. Conclusion: This is the first study to investigate an early marker of NE. NOS1 is elevated with hypoxia, and further studies are needed to investigate it as a valuable tool for early diagnosis of neonatal brain injury. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.335 2017 PEDIATRICS 38 / 124 = 0.306 2017 Q2 T1 0.0 2017 Pediatrics, Perinatology and Child Health 2017 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Paules, C. Nigrini, E. Rosenzweig, J.M. Bahabry, R. Farzin, A. Yang, S. Northington, F.J. Oros, D. Universidad de Zaragoza (orcid)0000-0002-8476-9161 McKenney, S. Johnston, M.V. Graham, E.M. Burd, I. 1004 645 Universidad de Zaragoza Dpto. Cirugía,Ginecol.Obstetr. Área Obstetricia y Ginecología 5 (2017), 112 Front. pediatr. FRONTIERS IN PEDIATRICS 2296-2360 301374 http://zaguan.unizar.es/record/70694/files/texto_completo.pdf Versión publicada 90819 http://zaguan.unizar.es/record/70694/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:70694 articulos driver 2022-06-21-09:40:01 ARTICLE