000070732 001__ 70732
000070732 005__ 20240122154813.0
000070732 0248_ $$2sideral$$a99903
000070732 037__ $$aART-2015-99903
000070732 041__ $$aeng
000070732 100__ $$0(orcid)0000-0001-5573-6144$$aLayunta, E.$$uUniversidad de Zaragoza
000070732 245__ $$aExpression of microbiota recognition receptors and intestinal serotoninergic system in two mouse models of colitis
000070732 260__ $$c2015
000070732 5060_ $$aAccess copy available to the general public$$fUnrestricted
000070732 5203_ $$aBackground: The intestinal microbiota patterns recognition receptors TLRs and NODs (PRRs), and the intestinal serotoninergic system, may contribute to intestinal responses to microbiota and alter intestinal homeostasis/inflammation e.g. in inflammatory bowel disease.
Aim: We examined two mouse colitis models (Dextran Sulfate Sodium (DSS) or Lymphocyte Transfer (LT) mouse colitis) and compared the expression of PRRs, implicated in innate immunity, and some elements of the intestinal serotoninergic system.
Methods: In ileum and colon from DSS or LT mouse colitis animals, TLRs, NODs, serotonin transporter (SERT) and serotonin receptors (5-HTRs) mRNA expression was measured by RT-qPCR. SERT protein expression was analyzed by western blotting.
Results: In DSS ileum, TLR9, 5HTR1A, 5-HTR4 and 5-HTR7 mRNA levels were over-expressed, and SERT expression reduced; in DSS colon, NOD2, TLR2, TLR9, and 5-HTR7 mRNAs were increased; however, 5HTR1A, 5-HTR2B, 5-HTR3, and 5HTR4 mRNA levels were diminished, as well as SERT expression. On the other hand, in LT ileum, TLR9, 5-HTR1A, 5- HT2A, and 5-HT2B mRNAs were reduced and, although SERT mRNA was not altered, SERT protein level was reduced; in colon of LT mouse model, TLR2, TLR9, 5-HTR1A, and 5-HTR7 mRNA levels and SERT expression were increased; however, TLR4, NOD1, 5- HTR2B, 5-HT3, 5-HT4 mRNA levels were reduced.
000070732 536__ $$9info:eu-repo/grantAgreement/ES/DGA/ARAINF-012-2008$$9info:eu-repo/grantAgreement/ES/DGA/B022-13$$9info:eu-repo/grantAgreement/ES/DGA/B105-11$$9info:eu-repo/grantAgreement/ES/DGA/B61$$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/BFU2009-08149$$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/BFU2010-18971$$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/SAF2011-22922$$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/SAF22812
000070732 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000070732 590__ $$a4.066$$b2015
000070732 591__ $$aPHYSIOLOGY$$b13 / 83 = 0.157$$c2015$$dQ1$$eT1
000070732 592__ $$a1.654$$b2015
000070732 593__ $$aPhysiology$$c2015$$dQ1
000070732 655_4 $$ainfo:eu-repo/semantics/conferenceObject$$vinfo:eu-repo/semantics/publishedVersion
000070732 700__ $$aLatorre, E.
000070732 700__ $$0(orcid)0000-0002-5306-9365$$aGrasa, L.$$uUniversidad de Zaragoza
000070732 700__ $$0(orcid)0000-0001-8584-3979$$aCastro, M.$$uUniversidad de Zaragoza
000070732 700__ $$0(orcid)0000-0002-7412-2073$$aPlaza, M.A.$$uUniversidad de Zaragoza
000070732 700__ $$aPardo, J.
000070732 700__ $$aSantiago, L.
000070732 700__ $$aMedina de Sánchez , F.
000070732 700__ $$aMartínez-Augustin, O.
000070732 700__ $$aOcón, B.
000070732 700__ $$aAranda, C.
000070732 700__ $$0(orcid)0000-0003-4758-3998$$aMesonero, J.E.$$uUniversidad de Zaragoza
000070732 700__ $$aAlcalde, A.I.
000070732 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología
000070732 773__ $$g214, Suppl. 701 (2015), 6 [I3]$$pActa Physiol.$$tActa Physiologica$$x1748-1708
000070732 8564_ $$s32611$$uhttps://zaguan.unizar.es/record/70732/files/texto_completo.pdf$$yVersión publicada
000070732 8564_ $$s119541$$uhttps://zaguan.unizar.es/record/70732/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000070732 909CO $$ooai:zaguan.unizar.es:70732$$particulos$$pdriver
000070732 951__ $$a2024-01-22-15:31:14
000070732 980__ $$aARTICLE