70815 20190709135426.0 doi 10.1016/j.cej.2016.11.126 sideral 97603 ART-2017-97603 eng (orcid)0000-0002-4019-4466 Valero, P. Universidad de Zaragoza Comparative effect of growth media on the monitoring of E. coli inactivation and regrowth after solar and photo-Fenton treatment 2017 Access copy available to the general public Unrestricted This study intends to assess the cultivability of photo-treated Escherichia coli K-12 on media with different selectivity (PCA, LBA, T-7, T-7 + TTC, VRBA and MAC) and to establish optimal conditions for bacterial recuperation. For these purposes, immediate and long-term bacterial recovery after SODIS and photo-Fenton are evaluated. Moreover, the use of catalase and sodium pyruvate supplements in the medium is studied. The non-selective medium PCA showed the highest counts for the untreated and treated cells due to its content in nutrients (e.g. glucose) and lack of inhibitors. On the contrary, the selective media showed lower recovery, being the culture media effectiveness: PCA > LBA > T-7 > T-7 + TTC > VRBA > MAC. The presence of inhibitors, such as heptadecyl sulfate in T-7 or bile salts in VRBA and MAC, reduced the cultivability of the treated cells. These compounds can probably diffuse into the cells more easily after SODIS and photo-Fenton as a consequence of the loss of the membrane integrity. In addition, the lack of yeast extract in MAC had a detrimental effect on E. coli recovery. Sodium pyruvate was tested as supplement to PCA, leading to slightly enhanced bacterial immediate recovery after SODIS, SODIS + H2O2 and photo-Fenton. The addition of catalase and sodium pyruvate to the bulk was studied as well, considerably increasing bacterial survival in the long-term due to their ability to neutralize residual H2O2. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 688928-WATERSPOUTT info:eu-repo/grantAgreement/EC/H2020/688928/EU/Water - Sustainable Point-Of-Use Treatment Technologies/WATERSPOUTT info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 6.735 2017 ENGINEERING, ENVIRONMENTAL 3 / 50 = 0.06 2017 Q1 T1 ENGINEERING, CHEMICAL 7 / 137 = 0.051 2017 Q1 T1 1.863 2017 Chemical Engineering (miscellaneous) 2017 Q1 Industrial and Manufacturing Engineering 2017 Q1 Environmental Chemistry 2017 Q1 Chemistry (miscellaneous) 2017 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Giannakis, S. (orcid)0000-0002-7456-4912 Mosteo, R. Universidad de Zaragoza (orcid)0000-0003-3014-0322 Ormad, M. P. Universidad de Zaragoza Pulgarin, C. 5005 790 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Tecnologi. Medio Ambiente 313 (2017), 109-120 Chem. eng. j. Chemical Engineering Journal 1385-8947 1695588 http://zaguan.unizar.es/record/70815/files/texto_completo.pdf Postprint 68078 http://zaguan.unizar.es/record/70815/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:70815 articulos driver 2019-07-09-11:29:41 ARTICLE