000070996 001__ 70996
000070996 005__ 20200428120341.0
000070996 0247_ $$2doi$$a10.1128/AEM.02538-17
000070996 0248_ $$2sideral$$a105414
000070996 037__ $$aART-2018-105414
000070996 041__ $$aeng
000070996 100__ $$aChueca, B.
000070996 245__ $$aWhole-genome sequencing and genetic analysis reveal novel stress responses to individual constituents of essential oils in Escherichia coli
000070996 260__ $$c2018
000070996 5060_ $$aAccess copy available to the general public$$fUnrestricted
000070996 5203_ $$aFood preservation by the use of essential oils (EOs) is being extensively studied because of the antimicrobial properties of their individual constituents (ICs). Three resistant mutants (termed CAR, CIT, and LIM) of Escherichia coli MG1655 were selected by subculturing with the ICs carvacrol, citral, and (+)-limonene oxide, respectively. These derivative strains showed increased MIC values of ICs and concomitantly enhanced resistance to various antibiotics (ampicillin, trimethoprim, chloramphenicol, tetracycline, kanamycin, novobiocin, norfloxacin, cephalexin, and nalidixic acid) compared to those for the parental strain (wild type [WT]). Whole-genome sequencing (WGS) of these hyperresistant strains permitted the identification of single nucleotide polymorphisms (SNPs) and deletions in comparison to the WT. In order to analyze the contribution of these mutations to the increased antimicrobial resistance detected in hyperresistant strains, derivative strains were constructed by allelic reversion. A role of the SoxR D137Y missense mutation in CAR was confirmed by growth in the presence of some ICs and antibiotics and by its tolerance to ICs but not to lethal heat treatments. In CIT, increased resistance relied on contributions by several detected SNPs, resulting in a frameshift in MarR and an in-frame GyrB ¿G157 mutation. Finally, both the insertion resulting in an AcrR frameshift and large chromosomal deletions found in LIM were correlated with the hyperresistant phenotype of this strain. The nature of the obtained mutants suggests intriguing links to cellular defense mechanisms previously implicated in antibiotic resistance.
000070996 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/AGL2012-32165$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2015-69565-P
000070996 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000070996 590__ $$a4.077$$b2018
000070996 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b33 / 162 = 0.204$$c2018$$dQ1$$eT1
000070996 591__ $$aMICROBIOLOGY$$b38 / 133 = 0.286$$c2018$$dQ2$$eT1
000070996 592__ $$a1.663$$b2018
000070996 593__ $$aApplied Microbiology and Biotechnology$$c2018$$dQ1
000070996 593__ $$aFood Science$$c2018$$dQ1
000070996 593__ $$aEcology$$c2018$$dQ1
000070996 593__ $$aBiotechnology$$c2018$$dQ1
000070996 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000070996 700__ $$aRenzoni, A.
000070996 700__ $$0(orcid)0000-0001-5053-8309$$aBerdejo, D.$$uUniversidad de Zaragoza
000070996 700__ $$0(orcid)0000-0002-0238-6328$$aPagán, R.$$uUniversidad de Zaragoza
000070996 700__ $$aKelley, W.L.
000070996 700__ $$0(orcid)0000-0002-7629-8101$$aGarcía-Gonzalo, D.$$uUniversidad de Zaragoza
000070996 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000070996 773__ $$g84, 7 (2018), e02538-17$$pAppl. environ. microbiol.$$tApplied and Environmental Microbiology$$x0099-2240
000070996 8564_ $$s381009$$uhttps://zaguan.unizar.es/record/70996/files/texto_completo.pdf$$yVersión publicada
000070996 8564_ $$s99752$$uhttps://zaguan.unizar.es/record/70996/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000070996 909CO $$ooai:zaguan.unizar.es:70996$$particulos$$pdriver
000070996 951__ $$a2020-04-28-12:01:24
000070996 980__ $$aARTICLE