70996 20200428120341.0 doi 10.1128/AEM.02538-17 sideral 105414 ART-2018-105414 eng Chueca, B. Whole-genome sequencing and genetic analysis reveal novel stress responses to individual constituents of essential oils in Escherichia coli 2018 Access copy available to the general public Unrestricted Food preservation by the use of essential oils (EOs) is being extensively studied because of the antimicrobial properties of their individual constituents (ICs). Three resistant mutants (termed CAR, CIT, and LIM) of Escherichia coli MG1655 were selected by subculturing with the ICs carvacrol, citral, and (+)-limonene oxide, respectively. These derivative strains showed increased MIC values of ICs and concomitantly enhanced resistance to various antibiotics (ampicillin, trimethoprim, chloramphenicol, tetracycline, kanamycin, novobiocin, norfloxacin, cephalexin, and nalidixic acid) compared to those for the parental strain (wild type [WT]). Whole-genome sequencing (WGS) of these hyperresistant strains permitted the identification of single nucleotide polymorphisms (SNPs) and deletions in comparison to the WT. In order to analyze the contribution of these mutations to the increased antimicrobial resistance detected in hyperresistant strains, derivative strains were constructed by allelic reversion. A role of the SoxR D137Y missense mutation in CAR was confirmed by growth in the presence of some ICs and antibiotics and by its tolerance to ICs but not to lethal heat treatments. In CIT, increased resistance relied on contributions by several detected SNPs, resulting in a frameshift in MarR and an in-frame GyrB ¿G157 mutation. Finally, both the insertion resulting in an AcrR frameshift and large chromosomal deletions found in LIM were correlated with the hyperresistant phenotype of this strain. The nature of the obtained mutants suggests intriguing links to cellular defense mechanisms previously implicated in antibiotic resistance. info:eu-repo/grantAgreement/ES/MINECO/AGL2012-32165 info:eu-repo/grantAgreement/ES/MINECO/AGL2015-69565-P info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 4.077 2018 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 33 / 162 = 0.204 2018 Q1 T1 MICROBIOLOGY 38 / 133 = 0.286 2018 Q2 T1 1.663 2018 Applied Microbiology and Biotechnology 2018 Q1 Food Science 2018 Q1 Ecology 2018 Q1 Biotechnology 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Renzoni, A. (orcid)0000-0001-5053-8309 Berdejo, D. Universidad de Zaragoza (orcid)0000-0002-0238-6328 Pagán, R. Universidad de Zaragoza Kelley, W.L. (orcid)0000-0002-7629-8101 García-Gonzalo, D. Universidad de Zaragoza 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 84, 7 (2018), e02538-17 Appl. environ. microbiol. Applied and Environmental Microbiology 0099-2240 381009 http://zaguan.unizar.es/record/70996/files/texto_completo.pdf Versión publicada 99752 http://zaguan.unizar.es/record/70996/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:70996 articulos driver 2020-04-28-12:01:24 ARTICLE