000071163 001__ 71163 000071163 005__ 20210310171107.0 000071163 0247_ $$2doi$$a10.1371/journal.pone.0199914 000071163 0248_ $$2sideral$$a106922 000071163 037__ $$aART-2018-106922 000071163 041__ $$aeng 000071163 100__ $$0(orcid)0000-0002-0827-110X$$aHedman, Carlos 000071163 245__ $$aDetection of PrPres in peripheral tissue in pigs with clinical disease induced by intracerebral challenge with sheep-passaged bovine spongiform encephalopathy agent 000071163 260__ $$c2018 000071163 5060_ $$aAccess copy available to the general public$$fUnrestricted 000071163 5203_ $$aBovine spongiform encephalopathy (BSE) can be efficiently transmitted to pigs via intrace- rebral inoculation. A clear link has been established between the consumption of products of bovine origin contaminated with the BSE agent and the development of variant Creutz- feldt-Jakob disease in humans. Small ruminants can also naturally develop BSE, and sheep-adapted BSE (Sh-BSE) propagates more efficiently than cattle BSE in pigs and in mouse models expressing porcine prion protein. In addition, Sh-BSE shows greater effi- ciency of transmission to human models than original cow BSE. While infectivity and/or abnormal PrP accumulation have been reported in the central nervous system in BSE- infected pigs, the ability of the agent to replicate in peripheral tissues has not been fully investigated. We previously characterized the presence of prions in a panel of tissues col- lected at the clinical stage of disease from pigs experimentally infected with Sh-BSE. West- ern blot revealed low levels of PrPres accumulation in lymphoid tissues, nerves, and skeletal muscles from 4 of the 5 animals analysed. Using protein misfolding cyclic amplification (PMCA), which we found to be 6 log fold more sensitive than direct WB for the detection of pig BSE, we confirmed the presence of the Sh-BSE agent in lymphoid organs, nerves, ileum, and striated muscles from all 5 inoculated pigs. Surprisingly, PrPres positivity was also detected in white blood cells from one pig using this method. The presence of infectivity in lymphoid tissues, striated muscles, and peripheral nerves was confirmed by bioassay in bovine PrP transgenic mice. These results demonstrate the ability of BSE-derived agents to replicate efficiently in various peripheral tissues in pigs. Although no prion transmission has been reported in pigs following oral BSE challenge, our data support the continuation of the Feed Ban measure implemented to prevent entry of the BSE agent into the feed chain. 000071163 536__ $$9info:eu-repo/grantAgreement/EUR/INTERREG-POCTEFA/EFA 282-13-TRANSPRION$$9info:eu-repo/grantAgreement/EUR/INTERREG-POCTEFA/EFA-148-16 REDPRION 000071163 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000071163 590__ $$a2.776$$b2018 000071163 591__ $$aMULTIDISCIPLINARY SCIENCES$$b23 / 69 = 0.333$$c2018$$dQ2$$eT2 000071163 592__ $$a1.1$$b2018 000071163 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2018$$dQ1 000071163 593__ $$aMedicine (miscellaneous)$$c2018$$dQ1 000071163 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2018$$dQ1 000071163 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000071163 700__ $$0(orcid)0000-0001-9075-2764$$aOtero, Alicia, Douet, Jean-Yves$$uUniversidad de Zaragoza 000071163 700__ $$aLacroux, Caroline 000071163 700__ $$aLugan, Séverine 000071163 700__ $$aFilali, Hicham 000071163 700__ $$0(orcid)0000-0003-3352-740X$$aCorbière, Fabien$$uUniversidad de Zaragoza 000071163 700__ $$aAron, Naima 000071163 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan José$$uUniversidad de Zaragoza 000071163 700__ $$aAndréoletti, Olivier 000071163 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, Rosa.$$uUniversidad de Zaragoza 000071163 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000071163 773__ $$g13, 7 (2018), 0199914 [14 pp]$$pPLoS One$$tPloS one$$x1932-6203 000071163 8564_ $$s5426180$$uhttps://zaguan.unizar.es/record/71163/files/texto_completo.pdf$$yVersión publicada 000071163 8564_ $$s109101$$uhttps://zaguan.unizar.es/record/71163/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000071163 909CO $$ooai:zaguan.unizar.es:71163$$particulos$$pdriver 000071163 951__ $$a2021-03-10-17:01:02 000071163 980__ $$aARTICLE