000071164 001__ 71164
000071164 005__ 20200217133052.0
000071164 0247_ $$2doi$$a10.3389/fnana.2018.00055
000071164 0248_ $$2sideral$$a106918
000071164 037__ $$aART-2018-106918
000071164 041__ $$aeng
000071164 100__ $$aFernández-Sánchez, Laura
000071164 245__ $$aRetinal Vascular Degeneration in the Transgenic P23H Rat Model of Retinitis Pigmentosa
000071164 260__ $$c2018
000071164 5060_ $$aAccess copy available to the general public$$fUnrestricted
000071164 5203_ $$aRetinitis pigmentosa (RP) is a group of inherited retinal degenerative diseases involving a progressive degeneration of photoreceptor cells. Following the loss of photoreceptors, retinal vascularization tends to decrease, which seems to play a role in the degenerative process of retinal cells. This study reports changes in retinal vascular network architecture in the P23H rat model of RP at different stages of retinal degeneration. Homozygous P23H line-3 rats of ages ranging from 18 days to 16 months were used in this study. Age-matched Sprague-Dawley (SD) rats were used as control animals. Vertical sections and wholemount retinas were immunolabeled for type IV collagen or stained using NADPH diaphorase histochemistry, and retinal vascular networks were drawn using a camera lucida. The superficial and deep capillary plexus (DCP) were fully developed at P18 in P23H rat retinas and showed no differences from the control animals. In 4-month-old P23H rat retinas, the superficial and intermediate capillary plexus were similar to those observed in age-matched SD rats, but a reduction in the DCP could be observed in these animals, with a significant decrease in both capillary density and capillary loops. At 16 months, the DCP was completely lost, and only vessels exhibiting an abnormal, tortuous dead-end could be observed. The middle capillary plexus had virtually disappeared at this age. Only perpendicular vessels connecting the superficial and DCP were found. The superficial plexus showed no changes in the vascular surface with age. In RP, photoreceptor loss is accompanied by degenerative changes in the retinal vascular network. The disruption of the capillary plexus, with loss of capillary density and capillary loops, can hamper the normal supply of oxygen and nutrients to retinal cells, thus accelerating retinal degeneration. Therefore, changes in retinal vascularization must be taken into account in the design of therapies targeting retinal degenerative diseases.
000071164 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/RD16-0008-0016$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/BFU2015-67139-R
000071164 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000071164 590__ $$a2.923$$b2018
000071164 591__ $$aANATOMY & MORPHOLOGY$$b2 / 21 = 0.095$$c2018$$dQ1$$eT1
000071164 591__ $$aNEUROSCIENCES$$b131 / 266 = 0.492$$c2018$$dQ2$$eT2
000071164 592__ $$a1.808$$b2018
000071164 593__ $$aAnatomy$$c2018$$dQ1
000071164 593__ $$aNeuroscience (miscellaneous)$$c2018$$dQ1
000071164 593__ $$aCellular and Molecular Neuroscience$$c2018$$dQ1
000071164 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000071164 700__ $$aEsquiva, Gema
000071164 700__ $$0(orcid)0000-0003-0349-9997$$aPinilla, Isabel$$uUniversidad de Zaragoza
000071164 700__ $$aLax, Pedro
000071164 700__ $$aCuenca, Nicolás
000071164 7102_ $$11004$$2646$$aUniversidad de Zaragoza$$bDpto. Cirugía,Ginecol.Obstetr.$$cÁrea Oftalmología
000071164 773__ $$g12 (2018), 55 1-14$$pFront. neuroanat.$$tFRONTIERS IN NEUROANATOMY$$x1662-5129
000071164 8564_ $$s8611993$$uhttps://zaguan.unizar.es/record/71164/files/texto_completo.pdf$$yVersión publicada
000071164 8564_ $$s11419$$uhttps://zaguan.unizar.es/record/71164/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000071164 909CO $$ooai:zaguan.unizar.es:71164$$particulos$$pdriver
000071164 951__ $$a2020-02-17-12:43:23
000071164 980__ $$aARTICLE