71187 20200117221603.0 doi 10.1038/s41598-018-28394-x sideral 106915 ART-2018-106915 eng (orcid)0000-0002-9250-9060 Segura, Francisco Universidad de Zaragoza Development of optokinetic tracking software for objective evaluation of visual function in rodents 2018 Access copy available to the general public Unrestricted The aim of this study was to develop software that performs the optokinetic tracking assessment without the involvement of experimenters to increase the objectivity of the test. To check the effectiveness of the software, several videos were analyzed and the results were compared to those produced by two experimenters. Videos consisted of visual acuity and contrast sensitivity tests on normal animals and pigmented P23H rats (animal model of retinitis pigmentosa). Our software showed a reasonably high success rate: in approximately 78% of the cases, both the software program and the experimenters were in agreement, including the direction of rotation. The software detected 7% false positive cases, 10% false negative cases, and it was wrong in 5% of the cases. Decrease in visual function with age in pigmented P23H rats was observed from the first time interval, although minimum thresholds were found in visual parameters at advanced ages. We developed simple software based on current functions included in the Matlab image processing toolbox that was able to recognize, with a reasonably high percentage of success, the subtle head movements of the rodent produced when visual perception of the optokinetic optotype occurs. info:eu-repo/grantAgreement/ES/DGA/B99 info:eu-repo/grantAgreement/ES/ISCIII/PI13-01124 info:eu-repo/grantAgreement/ES/ISCIII/RETICS-RD16-0008 info:eu-repo/grantAgreement/ES/ISCIII/RETICS-RD16-0016 info:eu-repo/grantAgreement/ES/MINECO/FIS2016-77319 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.011 2018 MULTIDISCIPLINARY SCIENCES 14 / 69 = 0.203 2018 Q1 T1 1.414 2018 Multidisciplinary 2018 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Arines, Justo (orcid)0000-0002-5621-1937 Sánchez-Cano, Ana Universidad de Zaragoza (orcid)0000-0002-9261-0302 Perdices, Lorena (orcid)0000-0003-2710-1875 Orduna-Hospital, Elvira Universidad de Zaragoza (orcid)0000-0003-2656-6750 Fuentes-Broto, Lorena Universidad de Zaragoza (orcid)0000-0003-0349-9997 Pinilla, Isabel Universidad de Zaragoza 1005 410 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Fisiología 1004 646 Universidad de Zaragoza Dpto. Cirugía,Ginecol.Obstetr. Área Oftalmología 2002 647 Universidad de Zaragoza Dpto. Física Aplicada Área Óptica 8, 1 (2018), 10009 [11 p.] Sci. rep. SCIENTIFIC REPORTS 2045-2322 2062634 http://zaguan.unizar.es/record/71187/files/texto_completo.pdf Versión publicada 110785 http://zaguan.unizar.es/record/71187/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:71187 articulos driver 2020-01-17-21:44:02 ARTICLE