000074862 001__ 74862 000074862 005__ 20200117211600.0 000074862 0247_ $$2doi$$a10.3389/fmicb.2018.01246 000074862 0248_ $$2sideral$$a107166 000074862 037__ $$aART-2018-107166 000074862 041__ $$aeng 000074862 100__ $$aVidal-García, M. 000074862 245__ $$aProduction of the invasive aspergillosis biomarker bis(methylthio)gliotoxin Within the genus aspergillus: In vitro and in vivo metabolite quantification and genomic analysis 000074862 260__ $$c2018 000074862 5060_ $$aAccess copy available to the general public$$fUnrestricted 000074862 5203_ $$aGliotoxin (GT) is a fungal secondary metabolite that has attracted great interest due to its high biological activity since it was discovered by the 1930s. An inactive derivative of this molecule, bis(methylthio)gliotoxin (bmGT), has been proposed as an invasive aspergillosis (IA) biomarker. Nevertheless, studies regarding bmGT production among common opportunistic fungi, including the Aspergillus genus, are scarce and sometimes discordant. As previously reported, bmGT is produced from GT by a methyl-transferase, named as GtmA, as a negative feedback regulatory system of GT production. In order to analyze the potential of bmGT detection to enable identification of infections caused by different members of the Aspergillus genus we have assessed bmGT production within the genus Aspergillus, including A, fumigatus, A. niger, A. nidulans, and A. flavus, and its correlation with gtmA presence. In order to validate the relevance of our in vitro findings, we compared bmGT during in vitro culture with the presence of bmGT in sera of patients from whom the Aspergillus spp. were isolated. Our results indicate that most A. fumigatus isolates produce GT and bmGT both in vitro and in vivo. In contrast, A. niger and A. nidulans were not able to produce GT or bmGT, although A. niger produced bmGT from a exogenous GT source. The frequency and amount of bmGT production in A. terreus and A. flavus isolates in vitro was lower than in A. fumigatus. Our results suggest that this defect could be related to the in vitro culture conditions, since isolates that did not produce bmGT in vitro were able to synthetize it in vivo. In summary, our study indicates that bmGT could be very useful to specifically detect the presence of A. fumigatus, the most prevalent agent causing IA. Concerning A. terreus and A. flavus a higher number of analyses from sera from infected patients will be required to reach a useful conclusion. 000074862 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/MAT2011-26851-CO2-02$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-1-R$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-2-R 000074862 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000074862 590__ $$a4.259$$b2018 000074862 591__ $$aMICROBIOLOGY$$b32 / 133 = 0.241$$c2018$$dQ1$$eT1 000074862 592__ $$a1.633$$b2018 000074862 593__ $$aMicrobiology (medical)$$c2018$$dQ1 000074862 593__ $$aMicrobiology$$c2018$$dQ1 000074862 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000074862 700__ $$aRedrado, S. 000074862 700__ $$aDomingo, M.P. 000074862 700__ $$aMarquina, P. 000074862 700__ $$aColmenarejo, C. 000074862 700__ $$aMeis, J.F. 000074862 700__ $$0(orcid)0000-0001-7294-245X$$aRezusta, A.$$uUniversidad de Zaragoza 000074862 700__ $$0(orcid)0000-0003-0154-0730$$aPardo, J.$$uUniversidad de Zaragoza 000074862 700__ $$aGalvez, E.M. 000074862 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Microbiología 000074862 7102_ $$11008$$2566$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Inmunología 000074862 773__ $$g9 (2018), 1426 [9 pp.]$$pFront. microbiol.$$tFRONTIERS IN MICROBIOLOGY$$x1664-302X 000074862 8564_ $$s792725$$uhttps://zaguan.unizar.es/record/74862/files/texto_completo.pdf$$yVersión publicada 000074862 8564_ $$s23841$$uhttps://zaguan.unizar.es/record/74862/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000074862 909CO $$ooai:zaguan.unizar.es:74862$$particulos$$pdriver 000074862 951__ $$a2020-01-17-21:11:03 000074862 980__ $$aARTICLE