000074920 001__ 74920
000074920 005__ 20200117221645.0
000074920 0247_ $$2doi$$a10.1042/BCJ20180172
000074920 0248_ $$2sideral$$a107715
000074920 037__ $$aART-2018-107715
000074920 041__ $$aeng
000074920 100__ $$aRaimi, O.G.
000074920 245__ $$aEvidence for substrate-assisted catalysis in N-acetylphosphoglucosamine mutase
000074920 260__ $$c2018
000074920 5060_ $$aAccess copy available to the general public$$fUnrestricted
000074920 5203_ $$aN-acetylphosphoglucosamine mutase (AGM1) is a key component of the hexosamine biosynthetic pathway that produces UDP-GlcNAc, an essential precursor for a wide range of glycans in eukaryotes. AGM belongs to the a-D-phosphohexomutase metalloenzyme superfamily and catalyzes the interconversion of N-acetylglucosamine-6-phosphate (GlcNAc-6P) to N-acetylglucosamine-1-phosphate (GlcNAc-1P) through N-acetylglucosa-mine-1, 6-bisphosphate (GlcNAc-1, 6-bisP) as the catalytic intermediate. Although there is an understanding of the phosphoserine-dependent catalytic mechanism at enzymatic and structural level, the identity of the requisite catalytic base in AGM1/phosphoglucomu-tases is as yet unknown. Here, we present crystal structures of a Michaelis complex of AGM1 with GlcNAc-6P and Mg2+, and a complex of the inactive Ser69Ala mutant together with glucose-1, 6-bisphosphate (Glc-1, 6-bisP) that represents key snapshots along the reaction co-ordinate. Together with mutagenesis, these structures reveal that the phosphate group of the hexose-1, 6-bisP intermediate may act as the catalytic base.
000074920 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000074920 590__ $$a4.331$$b2018
000074920 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b72 / 294 = 0.245$$c2018$$dQ1$$eT1
000074920 592__ $$a2.142$$b2018
000074920 593__ $$aBiochemistry$$c2018$$dQ1
000074920 593__ $$aMolecular Biology$$c2018$$dQ1
000074920 593__ $$aCell Biology$$c2018$$dQ1
000074920 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000074920 700__ $$0(orcid)0000-0002-3122-9401$$aHurtado-Guerrero, R.$$uUniversidad de Zaragoza
000074920 700__ $$aVan Aalten, D.M.F.
000074920 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000074920 773__ $$g475, 15 (2018), 2547-2557$$pBiochem. j.$$tBIOCHEMICAL JOURNAL$$x0264-6021
000074920 8564_ $$s569349$$uhttps://zaguan.unizar.es/record/74920/files/texto_completo.pdf$$yVersión publicada
000074920 8564_ $$s52632$$uhttps://zaguan.unizar.es/record/74920/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000074920 909CO $$ooai:zaguan.unizar.es:74920$$particulos$$pdriver
000074920 951__ $$a2020-01-17-22:06:19
000074920 980__ $$aARTICLE